Mycosis vaccines
AssigneesIgor Dimitrievich Poliakov, Ludmilla Ivanova
InventorsIgor Dimitrievich Poliakov, Ludmilla Ivanova
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Abstract
The present invention pertains to vaccines comprising homogenised inactivated yeast blastospores and homogenised inactivated dermatophyte microconidia or antigenic material of said spores, methods for their production and their use for the prophylaxis and/or treatment of mycoses in mammals, preferably humans. The vaccines according to the present invention are especially useful for the prophylaxis and/or treatment of skin mycosis, preferably Dermatomycosis and/or Candidosis and/or Onychomycosis.
Claims32
A vaccine comprising homogenized inactivated dermatophyte microconidia and inactivated yeast blastospores or antigenic material thereof.
A method for the preparation of a vaccine comprising:
- (a) growing a dermatophyte on a suitable solid medium and harvesting and homogenizing the dermatophyte microconidia or antigenic material therof;
- (b) growing a yeast on a suitable medium and harvesting and homogenizing the yeast blastopores or antigenic material therof;
- and
- (c) combining and inactivating the homogenate obtained in steps (a) and (b).
A method for the preparation of an increased amount of swollen microconidlia and microconidia with germ tubes of dermatophytes comprising:
- (a) cultivating a dermatophyte on a solid medium;
- (b) harvesting and homogenizing the culture in a liquid medium;
- (c) maintaining the pH of the liquid medium at 6.2 to 7.2;
- (d) transferring the suspension in a separate vessel containing fresh liquid medium;
- (e) monitoring the growth and morphological appearance of the dermatophyte cells;
- and
- (f) harvesting the cells when no less than 50% of the microconidia display a swollen or germinating condition, and no more than 7-10% of the cells display a second mycelial branch.
A method for the preparation of an increased amount of swollen blastospores and blastospores with germ tubes of yeast comprising:
- (a) cultivating yeast on a solid medium;
- (b) harvesting and homogenizing the yeast in a liquid medium;
- (c) incubating the homogenate in a CO2 atmosphere of 5-6% at 36-38° C. for 2-4 hours,
- (d) monitoring the growth and morphological appearance of the yeast cells;
- and
- (e) harvesting the cells when no less than 50% of the blastospores display germ tubes or a swollen condition.
Dependencies are inferred from the claim text.