Polynucleotides encoding an undercaprenyl diphosphate synthase of staphylococcus aureus
AssigneeSmithKline Beecham Corporation
InventorsJianzhong Huang, Xinhe Jiang, Damien McDevitt, Stephanie Van Horn
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Abstract
The invention provides ups polypedes and polynucleotides encoding ups polypeptides and methods for producing such polypeptides by recombinant techniques. Also provided are methods for utilizing ups polypeptides to screen for antibacterial compounds.
Claims21
An isolated polynucleotide comprising a polynucleotide sequence or the full complement of the entire length of the polynucleotide sequence, wherein the polynucleotide sequence is identical to SEQ ID NO:1, except that, over the entire length corresponding to SEQ ID NO:1, nn nucleotides are substituted, inserted or deleted, wherein nn satisfies the following expression
- nn≦xn−(xn·y)
- wherein xn is the total number of nucleotides in SEQ ID NO:1, y is at least 0.95, and wherein any non-integer product of xn and y is rounded down to the nearest integer before subtracting the product from xn;
- and wherein the polynucleotide sequence detects Staphylococcus aureus by hybridization and wherein the polynucleotide sequence is not genomic DNA.
An isolated polynucleotide comprising a polynucleotide sequence or the full complement of the entire length of the polynucleotide sequence, wherein the polynucleotide sequence comprises SEQ ID NO:1:
- wherein the polynucleotide sequence detects Staphylococcus aureus by hybridization and wherein the polynucleotide sequence is not genomic DNA.
An isolated polynucleotide comprising a polynucleotide sequence or the full complement of the entire length of the polynucleotide sequence, wherein the polynucleotide sequence hybridizes to the full complement of SEQ ID NO:1, wherein the hybridization conditions include incubation at 42° C. in a solution comprising:
- 50% formamide, 5×SSC (150 mM NaCl, 15 mM trisodium citrate), 50 mM sodium phosphate (pH7.6), 5×Denhardt's solution, 10% dextran sulfate, and 20 micrograms/ml denatured, sheared salmon sperm DNA, followed by washing in 0.1×SSC at 65° C.;
- and, wherein the polynucleotide sequence is identical to SEQ ID NO:1, except that, over the entire length corresponding to SEQ ID NO:1, nn nucleotides are substituted, inserted or deleted, wherein nn satisfies the following expression
- nn≦xn−(xn·y)
- wherein xn is the total number of nucleotides in SEQ ID NO:1, y is at least 0.95, and wherein any non-integer product of xn and y is rounded down to the nearest integer before subtracting the product from xn;
- and wherein the polynucleotide sequence detects Staphylococcus aureus by hybridization and wherein the polynucleotide sequence is not genomic DNA.
An isolated polynucleotide comprising a polynucleotide sequence, wherein the polynucleotide encodes a polypeptide comprising SEQ ID NO:2, wherein the polynucleotide sequence is not genomic DNA.
An isolated polynucleotide comprising a polynucleotide sequence, wherein the polynucleotide encodes a polypeptide consisting of SEQ ID NO:2;
- wherein the polynucleotide sequence is not genomic DNA.
Dependencies are inferred from the claim text.