Transgenic plants with divergent [ScaM4 or] SCaM5 gene to achieve multiple disease resistance
AssigneeKorea Kumho Petrochemical Co., Ltd.
InventorsWon Do Heo, Moo Je Cho, Pill-Soon Song, Chang Ho Chung
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Abstract
The present invention provides transgenic plants and plant cells thereof which have been transformed with the soybean calmodulin isoform (SCaM5) gene to exhibit greatly enhanced resistance to a wide spectrum of plant pathogens. The present invention also provides the expression vector containing SCaM5 gene and to host cells into which the gene in the expression vector has been introduced to plant pathogens-resistant plants. Transgenic plants expressing a heterologous SCaM5 gene show increased resistance to fungi, bacteria and viruses which normally infect the plants.
Claims26
A binary vector comprising a nucleotide sequence encoding ScaM5 protein (SEQ ID NO:4) and regulatory elements for the expression of ScaM5 in plant cells wherein the regulatory elements comprise a promoter and a transcription termination sequence.
The binary vector pLES9805.
A transgenic plant cell which is transformed with and expresses a gene encoding soybean calmodulin 5 (Scam5) protein (SEQ ID NO:4), or the transgenic progeny of said plant cell.
A transgenic plant, the cells of which express the gene contained in the vector pLES9805 (KCTC Accession No. 0546BP) encoding ScaM5 or the transgenic progeny of said plant.
A multiple disease resistant transgenic plant which contains in its genome a structural gene comprising in operable linkage:
- (i) a promoter functional in plant cells to cause the production of an RNA sequence,
- (ii) a nucleic acid sequence encoding a soybean calmodulin 5 (SEQ ID NO:4) protein;
- and
- (iii) a 3′ non-translated region which functions in plant cells to cause the termination of transcription and the addition of polyadenylated ribonucleotides to the 3′ end of transcribed mRNA sequences.
A method for enhancing multiple disease resistance of a higher plant to attack by one or more plant pathogens by transforming said plant with a gene encoding ScaM5(SEQ ID NO:4) to produce a transformed plant, wherein said transformed plant has enhanced multiple disease resistance as compared to an untransformed plant.
A method for enhancing multiple disease resistance of a plant wherein said plant is susceptible to plant pathogens comprising the steps of:
- (a) transforming plant cells with a chimeric recombinant DNA molecule comprising in operable linkage:
- (i) a promoter functional in plant cells to cause the production of an RNA sequence;
- (ii) a structural gene encoding a soybean calmodulin 5 (SEQ ID NO:4) protein;
- and
- (iii) a 3′ non-translated region which functions in plant cells to cause the termination of transcription and the addition of polyadenylated ribonucleotides to the 3′ end of transcribed MRNA sequences;
- and
- (b) regenerating said plant cells to provide a differentiated plant, wherein said differentiated plant has enhanced multiple disease resistance as compared to an untransformed plant.
Dependencies are inferred from the claim text.