Insect-resistant plants comprising a Bacillus thuringiensis gene
AssigneeMonsanto Company
InventorsDavid A. Fischhoff, Roy L. Fuchs, Paul B. Lavrik, Sylvia A. McPherson, Frederick J. Perlak
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Abstract
A method for producing genetically transformed plants exhibiting toxicity to Coleopteran insects is disclosed. In another aspect, the present invention embraces chimeric plant genes, genetically transformed cells and differentiated plants which exhibit toxicity to Coleopteran insects. In yet another aspect, the present invention embraces bacterial cells and plant transformation vectors comprising a chimeric plant gene encoding a Coleopteran toxin protein of Bacillus thuringiensis.
Claims13
A chimeric plant gene comprising in sequence:
- (a) a promoter which functions in plants to cause the production of RNA;
- (b) a DNA sequence encoding SEQ ID NO:2 or an insecticidally active fragment thereof;
- and
- (c) a 3′ non-translated DNA sequence which functions in plant cells to cause the addition of polyadenylate nucleotides to the 3′ end of an RNA sequence.
A plant transformation vector comprising in sequence:
- (a) a promoter which functions in plants to cause the production of an RNA sequence;
- (b) a DNA sequence encoding SEQ ID NO:2 or an insecticidally active fragment thereof;
- and
- (c) a 3′ non-translated DNA sequence which functions in plant cells to cause the addition of polyadenylate nucleotides to the 3′ end of an RNA sequence.
A transformed plant cell comprising a chimeric gene comprising in sequence:
- (a) a promoter which functions in plants to cause the production of RNA;
- (b) a DNA sequence that causes the production of an RNA sequence encoding SEQ ID NO:2 or an insecticidally active fragment thereof;
- and
- (c) a 3′ non-translated DNA sequence which functions in plant cells to cause the addition of polyadenylate nucleotides to the 3′ end of an RNA sequence;
- wherein the plant cell exhibits toxicity toward susceptible Coleopteran insects.
A differentiated plant exhibiting toxicity toward susceptible Coleopteran insects, the plant comprising transformed plant cells, the transformed plant cells comprising in sequence:
- (a) a promoter which functions in plants to cause the production of an RNA sequence;
- (b) a DNA sequence encoding SEQ ID NO:2 or an insecticidally active fragment thereof;
- and
- (c) a 3′ non-translated DNA sequence which functions in plant cells to cause the addition of polyadenylate nucleotides to the 3′ end of an RNA sequence.
Dependencies are inferred from the claim text.