Methods of using viral replicase polynucleotides and polypeptides
AssigneePioneer Hi-Bred International, Inc.
InventorsWilliam J. Gordon-Kamm, Keith S. Lowe, Matthew A. Bailey, Carolyn A. Gregory, George J. Hoerster, Brian A. Larkins, Brian R. Dilkes, Ronald Burnett, Young Min Woo
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Abstract
The invention provides novel methods of using viral replicase polypeptides and polynucleotides. Included are methods for increasing transformation frequencies, increasing crop yield, providing a positive growth advantage, modulating cell division, transiently modulating cell division, and for providing a means of positive selection.
Claims20
A method for increasing transformation frequency of a plant cell comprising introducing into a plant cell an isolated plant geminivirus replicase polynucleotide to produce a transformed plant cell, and growing the transformed plant cell under conditions sufficient to express the replicase polynucleotide, wherein the replicase polynucleotide is operably linked to a promoter capable of driving expression in the transformed plant cell.
A method for increasing crop yield comprising stably transforming a plant cell with an isolated plant geminivirus replicase polynucleotide operably linked to a promoter capable of driving expression in the plant cell, growing the plant cell under conditions sufficient to express the geminivirus polynucleotide, and regenerating a plant having increased yield compared to a corresponding plant that has not been transformed with the geminivirus polynucleotide.
A method for providing a positive growth advantage in a plant cell comprising introducing into the plant cell an isolated plant geminivirus replicase polynucleotide operably linked to a promoter capable of driving expression in the plant cell and growing the plant cell under conditions sufficient to express the polynucleotide.
A method for providing a means of positive selection comprising introducing into a plant cell an isolated plant geminivirus replicase polynucleotide, growing the cells under conditions sufficient to express the polynucleotide and selecting for cells exhibiting a positive growth advantage.
Dependencies are inferred from the claim text.