Feline Fc epsilon receptor alpha chain nucleic acid molecules
AssigneeHeska Corporation
InventorsGlenn R. Frank, James P. Porter, Keith E. Rushlow, Donald L. Wassom, Eric R. Weber
Life of this patent
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Abstract
The present invention relates to feline Fc epsilon receptor alpha chain nucleic acid molecules, compositions comprising such nucleic acid molecules, and methods to use such nucleic acid molecules to produce feline Fc epsilon receptor alpha chain proteins.
Claims17
An isolated feline nucleic acid molecule selected from the group consisting of:
- (a) a feline nucleic acid molecule that encodes a protein that binds to IgE and hybridizes to a nucleic acid sequence selected from the group consisting of SEQ ID NO:3, SEQ ID NO:5, SEQ ID NO:8, SEQ ID NO:15 and SEQ ID NO:16 under hybridization and wash conditions which allow 30% or less base-pair mismatch, wherein such conditions are determined by a formula:
- Tm=81.5° C.+16.6 log M+0.41 (% G+C)−500/n−0.61 (% formamide),
- wherein Tm represents the temperature at which two complementary nucleic acid molecule strands will disassociate, assuming 100% complementarity between the two strands, n represents the number of nucleotides in the shorter strand of the duplex being hybridized and log M represents the ionic strengths of the hybridization and wash solutions in moles/liter;
- wherein said wash is conducted at a temperature of Tm minus 30° C.;
- and
- (b) a feline nucleic acid molecule fully complementary to a nucleic acid molecule of (a).
An isolated nucleic acid molecule comprising a nucleic acid sequence selected from the group consisting of SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:8, SEQ ID NO:11, SEQ ID NO:14, SEQ ID NO:15 and SEQ ID NO:16.
A method to produce a feline FcεRα protein, said method comprising culturing a cell transformed with a feline nucleic acid molecule, wherein said feline nucleic acid molecule encodes a protein that binds to IgE and hybridizes to a nucleic acid sequence selected from the group consisting of SEQ ID NO:3, SEQ ID NO:5, SEQ ID NO:8, SEQ ID NO:15 and SEQ ID NO:16 under hybridization and wash conditions which allow 30% or less base-pair mismatch, wherein such conditions are determined by a formula:
- Tm=81.5° C.+16.6 log M+0.41(% G+C)−500/n−0.61(% formamide),
- wherein Tm represents the temperature at which two complementary nucleic acid molecule strands will disassociate, assuming 100% complementarity between the two strands, n represents the number of nucleotides in the shorter strand of the duplex being hybridized and log M represents the ionic strengths of the hybridization and wash solutions in moles/liter;
- wherein said wash is conducted at a temperature of Tm minus 30° C.
A composition comprising an excipient and a feline nucleic acid molecule selected from the group consisting of:
- (a) a feline nucleic acid molecule that encodes a protein that binds to IgE and hybridizes to a nucleic acid sequence selected from the group consisting of SEQ ID NO:3, SEQ ID NO:5, SEQ ID NO:8, SEQ ID NO:15 and SEQ ID NO:16 under hybridization and wash conditions which allow 30% or less base-pair mismatch, wherein such conditions are determined by a formula:
- Tm=81.5° C.+16.6 log M+0.41(% G+C)−500/n−0.61(% formamide),
- wherein Tm represents the temperature at which two complementary nucleic acid molecule strands will disassociate, assuming 100% complementarity between the two strands, n represents the number of nucleotides in the shorter strand of the duplex being hybridized and log M represents the ionic strengths of the hybridization and wash solutions in moles/liter;
- wherein said wash is conducted at a temperature of Tm minus 30° C.;
- and
- (b) a nucleic acid sequence fully complementary to a nucleic acid sequence of (a).
Dependencies are inferred from the claim text.