Rapid papanicolaou staining method for cervico-vaginal specimens
OwnerIliana I. Alvarezinventor
Life of this patent
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Abstract
A method for staining a Pap test slide specimen for conventional Pap test and, particularly a ThinPrep Pap test specimen, in which a number of multiple hydrating, staining and dehydrating step are performed in an equal number of 10 to 15 dippings at about equal dip times of about 1 second. The total method time is 100 to 150 seconds. The specimen pre-staining fixative solution is a 10% buffered formalin solution and a 95% lower alcohol in a 1:2 volumetric ratio. The method simplifies the staining process and laboratory technician error, while providing an improved stain contrast with a clean background in the resultant conventional and ThinPrep Pap test stained specimens.
Claims17
A method for staining a gynecological Pap test slide specimen comprising:
- (a) fixing the slide specimen;
- (b) hydrating the slide specimen from step (a);
- (c) staining the slide specimen from step (b) for at least about 10 to 15 seconds;
- (d) hydrating the slide specimen from step (c);
- (e) staining the slide specimen from step (d) for at least about 10 to 15 seconds;
- and
- (f) dehydrating the slide specimen from step (e);
- wherein the total time for steps (a) through (f) is no more than about 100 to 150 seconds.
A method for staining a Pap test slide specimen comprising;
- (a) fixing the slide specimen;
- (b) hydrating the slide specimen from step (a);
- (c) staining the slide specimen from step (b) with hematoxylin;
- (d) hydrating the slide specimen from step (c);
- (e) staining the slide specimen from step (d) with cytostain;
- and
- (f) dehydrating the slide specimen from step (e);
- wherein steps (a) through (e) are about equal times of 10 to 15 seconds.
A method for staining a gynecological Pap test slide specimen comprising:
- (a) fixing the slide specimen;
- (b) hydrating the slide specimen from step (a);
- (c) staining the slide specimen from step (b);
- (d) hydrating the slide specimen from step (c);
- (e) staining the slide specimen (d);
- and
- (f) dehydrating the slide specimen from step (e) in three steps (f) (i)-(f) (iii);
- wherein each step (a) through (f)(iii) is for about the same time.
Dependencies are inferred from the claim text.