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US 6,284,525 B1Granted
Miniature reaction chamber and devices incorporating same
AssigneeAffymetrix, Inc.
InventorsRichard A. Mathies, Adam T. Woolley
Life of this patent
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Abstract
The present invention generally relates to miniaturized devices for carrying out and controlling chemical reactions and analyses. In particular, the present invention provides devices which have miniature temperature controlled reaction chambers for carrying out a variety of synthetic and diagnostic applications, such as PCR amplification, nucleic acid hybridization, chemical labeling, nucleic acid fragmentation and the like.
Claims36
A miniature reaction system comprising:
- a body having a cavity disposed therein;
- effective amounts of reaction components disposed within said cavity;
- a heater deposited within said cavity, said heater being electrically connected to a power source;
- a temperature sensor in thermal contact with said cavity;
- an appropriately programmed computer for monitoring said temperature and controlling said power source;
- and
- a microcapillary gel electrophoresis device comprising a first gel filled microcapillary extending from the cavity, and a second gel filed microcapillary intersecting across the first gel filled microcapillary, the second gel filled microcapillary being in fluid communication with the first gel filled microcapillary, said second gel filled microcapillary comprising an electrophoretic channel.
4 more elements
A miniature reaction system comprising:
- a body having a cavity disposed therein;
- a heater deposited within said cavity, said heater being electrically connected to a power source;
- a temperature sensor in thermal contact with said cavity;
- an appropriately programmed computer for monitoring said temperature and controlling said power source;
- and
- a microcapillary gel electrophoresis device comprising a first gel filled microcapillary extending from the cavity, and a plurality of gel filed microcapillaries intersecting across the first gel filled microcapillary, the plurality of gel filled microcapillaries being in fluid communication with the first gel filled microcapillary, said plurality of gel filled microcapillaries comprising electrophoretic channels.
3 more elements
A miniature reaction system comprising:
- a body having a cavity disposed therein;
- a heater deposited within said cavity, said heater being electrically connected to a power source;
- a temperature sensor in thermal contact with said cavity;
- an appropriately programmed computer for monitoring said temperature and controlling said power source;
- and
- a microcapillary gel electrophoresis device comprising a first gel filled microcapillary extending from the cavity, and a second gel filled microcapillary intersecting across the first gel filled microcapillary, the second gel filled microcapillary being in fluid communication with the first gel filled microcapillary, said second gel filled microcapillary comprising an electrophoretic channel.
3 more elements
A miniature reaction system comprising:
- a body having a cavity disposed therein;
- a heater deposited within said cavity, said heater being electrically connected to a power source;
- a temperature sensor in thermal contact with said cavity;
- an appropriately programmed computer for monitoring said temperature and controlling said power source;
- a gel filled microcapillary extending from the cavity;
- and
- a reaction chamber in fluid communication with the gel filled microcapillary, the reaction chamber having a nucleic acid array disposed therein, said nucleic acid array including a substrate having a plurality of positionally distinct nucleic acid probes coupled to a surface of said substrate.
4 more elements
Dependencies are inferred from the claim text.