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US 6,284,522 B1Granted
Isolated and purified DNA molecule and protein for the degradation of triazine compounds
AssigneeRegents of the University of Minnesota
InventorsLawrence P. Wackett, Michael J. Sadowsky, Mervyn L. de Souza
Life of this patent
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Abstract
An isolated and purified DNA molecule, and an isolated and purified protein, that are involved in the degradation of s-triazine compounds (e.g., atrazine) are provided. A method for the purification of this protein is also provided.
Claims8
An isolated and purified DNA molecule encoding atrazine chlorohydrolase;
- the DNA molecule hybridizes to DNA complementary to DNA having the sequence shown in FIG. 6 (SEQ ID NO:1), beginning at position 236 and ending at position 1655, under the stringency conditions of hybridization in buffer containing 0.25 M Na2HPO4, 7% SDS, 1% BSA, 1.0 mM EDTA at 65° C., followed by washing with 0.1% SDS and 0.1×SSC at 65° C.
Dependencies are inferred from the claim text.