Synthetic media for the production of malolactic starter cultures
AssigneeCornell Research Foundation, Inc.
InventorsThomas Henick-Kling, Sibylle Krieger
Life of this patent
Estimating the term…
Abstract
This invention relates to the production of Leuconostoc oenos or Lactobacillus spp., preferably Lactobacillus plantarum, Lactobacillus hilgardii, Lactobacillus brevis or Lactobacillus casei biomass in novel synthetic media (which are free of fruit or vegetable or other natural juices). A key feature of the invention is the use of a fructose/glucose mixture where fructose is the primary carbohydrate source, as opposed to the more typical use of glucose or lactose. Fructose/glucose mixtures containing between 3% to about 45% glucose can be employed, preferably the amount is between about 5% and about 40% glucose. Most preferably, the amount of glucose is less then 20% of the mixture.
Claims18
A culture medium for a starter culture for winemaking involving malolactic fermentation, said culture medium being a synthetic non-vegetable juice containing medium comprising on an aqueous liquid medium basis:
- (a) from about 4 to about 6% of a sole carbohydrate source consisting essentially of fructose and glucose and containing on a fructose plus glucose basis from about 97% to about 55% fructose and from about 3% to about 45% glucose,
- (b) from about 0.19% to about 0.7% L-malic acid or salt thereof,
- (c) malolactic bacteria growth supporting amount of a nitrogen source,
- (d) malolactic bacteria growth supporting amount of essential minerals and vitamins.
A culture medium for a starter culture for winemaking involving malolactic fermentation, said culture medium being a synthetic non-vegetable juice containing medium comprising on an aqueous liquid medium basis:
- (a) from about 4 to about 6% of a sole carbohydrate source consisting essentially of fructose and glucose and containing on a fructose plus glucose basis from about 97% to about 50% fructose and from about 3% to about 50% glucose,
- (b) from about 0.19% to about 0.7% L-malic acid or salt thereof,
- (c) malolactic bacteria growth supporting amount of a nitrogen source,
- (d) malolactic bacteria growth supporting amount of essential minerals and vitamins.
A culture medium for a starter culture for winemaking involving malolactic fermentation, said culture medium being a synthetic non-vegetable juice containing medium comprising on an aqueous liquid medium basis:
- (a) from about 30 g/L to about 60 g/L of a sole carbohydrate source consisting essentially of fructose and glucose and including glucose in an amount providing at least 5 g/L of glucose and including fructose in an amount proving at least 15 g/L of fructose;
- (b) from about 0.19% to about 0.7% L-malic acid or salt thereof,
- (c) malolactic bacteria growth supporting amount of a nitrogen source,
- (d) malolactic bacteria growth supporting amount of essential minerals and vitamins.
Dependencies are inferred from the claim text.