Plating media for the presumptive identification of Bacillus cereus and Bacillus thuringiensis
OwnerLawrence Restainoinventor
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Abstract
An isolation plating medium and mixture for simultaneously identifying Bacillus species are disclosed. The specific bacilli identified are Bacillus thuringiensis and Bacillus cereus. The medium and mixture contain nutrients, inhibitory ingredients to inhibit the growth of other bacteria yeast and molds and a chromogenic substrate. The substrate changes color in response to the production of phosphatidylinositol-specific phospholipase C for the identification of the bacteria.
Claims14
Isolation plating medium for the simultaneous identification of Bacillus cereus and Bacillus thuringiensis consisting essentially of a mixture of (1) nutrients that promote growth of cells of Bacillus cereus and Bacillus thuringiensis under suitable environmental conditions for growth, (2) an ingredient that facilitates resuscitation of damaged Bacillus cereus and Bacillus thuringiensis cells under suitable environmental conditions for growth, (3) an ingredient that promotes germination of Bacillus spores under suitable environmental conditions for growth, (4) an ingredient that inhibits the growth of strains of related bacteria and Bacillus other than Bacillus cereus or Bacillus thuringiensis under suitable environmental conditions for growth, (5) an ingredient that inhibits the growth of yeast and molds, (6) a chromogenic substrate that changes color responsive to the presence of phosphatidylinositol-specific phospholipase C, and (7) an ingredient for thickening the mixture in sufficient quantity to solidify the mixture.
Isolation plating medium for the simultaneous identification of Bacillus cereus and Bacillus thuringiensis consisting essentially of a mixture of (1) nutrients that promote growth of cells of Bacillus cereus and Bacillus thuringiensis under suitable environmental conditions for growth consisting of proteose peptone, meat extract powder, and yeast extract, (2) an ingredient that facilitates resuscitation of damaged Bacillus cereus and Bacillus thuringiensis cells consisting of sodium pyruvate, (3) an ingredient that promotes germination of Bacillus spores consisting of magnesium sulfate, (4) an ingredient that inhibits the growth of related bacteria and strains of Bacillus other than Bacillus cereus or Bacillus thuringiensis under suitable environmental conditions for growth consisting of lithium chloride, ceftazidime and polymixin B sulfate, (5) an ingredient that inhibits the growth of yeast and molds consisting of cycloheximide, (6) a chromogenic substrate consisting of 5-bromo-4-chloro-3-indoxyl-myo-inositol-1-phosphate and that changes color responsive to the presence of phosphatidylinositol-specific phospholipase C, and (7) agar in sufficient quantity to solidify the mixture.
A mixture for the simultaneous identification of Bacillus cereus and Bacillus thuringiensis consisting essentially of (1) nutrients that promote growth of cells of Bacillus cereus and Bacillus thuringiensis under suitable environmental conditions for growth, (2) an ingredient that inhibits the growth of strains of related bacteria and Bacillus other than Bacillus cereus or Bacillus thuringiensis under suitable environmental conditions for growth, (3) an ingredient that inhibits the growth of yeast and molds, and (4) a chromogenic substrate that changes color responsive to the presence of phosphatidylinositol-specific phospholipase C.
Dependencies are inferred from the claim text.