DNA segments and methods for increasing polysaccharide production
AssigneesShin-Etsu Bio, Inc., Shin-Etsu Chemical Co., Ltd.
InventorsThomas J. Pollock, Motohide Yamazaki, Linda Thorne, Marcia Mikolajczak, Richard W. Armentrout
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Abstract
The present invention relates to DNA segments isolated from Sphingomonas sp. and involved in the biosynthetic production of sphingan polysaccharides to increase the production of the polysaccharide in engineered microorganisms. The present invention also relates to methods of engineering strains of Sphingomonas to produce bacteria which are hyperproducers of sphingan, methods of identifying and utilizing DNA fragments useful to enhance production of sphingan in bacteria and the hyperproducer bacteria.
Claims2
An isolated DNA sequence encoding the SpsB protein of Sphingomonas strain ATCC31554 wherein said DNA sequence is set forth in SEQ ID NO:2.
A biologically pure culture of a Sphingomonas microorganism ATCC 69744.
Dependencies are inferred from the claim text.