Process for the production of stromelysin catalytic domain protein
AssigneeWarner-Lambert Company
InventorsQi-Zhuang Ye, Linda Lea Johnson, Donald John Hupe, Vijaykumar Baragi
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Abstract
A process for the production of the catalytic domain, without propeptide, of a matrix metalloproteinase is described which comprises culturing transformed host cells carrying a DNA sequence encoding the catalytic domain as well as a method for screening for inhibitors of a matrix metalloproteinase; a method for determining the 3-dimensional structure of the catalytic domain of a matrix metalloproteinase; and pharmaceutical compositions of human stromelysin catalytic domain protein which are useful in treating herniated vertebral discs, dermal ulcers, modifying scar tissue formation, and joint diseases.
Claims9
A one step in vivo process for the production of a catalytic domain, without propeptide, of human stromelysin, consisting of culturing transformed Escherichia coli host cells comprising a DNA sequence encoding said catalytic domain.
A plasmid pGEMEX-D capable of expressing a human stromelysin catalytic domain protein which is constructed by inserting a cDNA fragment encoding said human stromelysin catalytic domain protein into the plasmid depicted in FIG. 6.
Dependencies are inferred from the claim text.