Heat-stable prolylendopeptidase
AssigneeJapat Ltd.
InventorsTetsuya Inaoka, Toyomi Ohkuma-Soyejima, Toshio Kokubo
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Abstract
The present invention relates to the field of biotechnology and concerns heat-stable prolylendopeptidase, recombinant DNA coding for heat-stable prolylendopeptidase, and processes for the production of heat-stable prolylendopeptidase and of recombinant DNA coding therefor, a host transformed with said recombinant DNA and a process for the production of said transformed host.
Claims24
A recombinant DNA molecule comprising a DNA sequence coding for a heat stable prolylendopeptidase, wherein said DNA sequence is derived by random mutation of a DNA sequence coding for a wild-type prolylendopeptidase of F. meningosepticum strain IFO 12535 (ATCC 13253) by a method comprising:
- (a) mutagenization of a starting DNA coding for a prolylendopeptidase:
- (b) generation of a library of mutated DNA sequences obtained in (a):
- and
- (c) screening the library for a gene coding for a prolylendopeptidase with improved heat stability.
Dependencies are inferred from the claim text.