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US 6,284,508 B1Granted
Glucuronoxylomannan (GXM)-O-acetylhydrolase of Cryptococcus neoformans and uses thereof
AssigneeResearch Development Foundation
InventorsAnne C. Savoy, Sherri L. Bloomer, Thomas R. Kozel
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Abstract
The present invention provides a novel enzyme which de-O-acetylates glucuronoxylomannan of Cryptococcus neoformans and a gene encoding such enzyme. Also provided are applications of such enzyme in treating cryptococcosis.
Claims5
Isolated and purified glucuronoxylomannan-O-acetylhydrolase coded for by DNA selected from the group consisting of:
- (a) isolated DNA which encodes glucuronoxylomannan-O-acetylhydrolase;
- (b) isolated DNA which hybridizes to isolated DNA of (a) above under high stringency conditions consisting of hybridization at 65° C. in 1.5×SSPE (3.0 M NaCl, 0.2 M NaH2PO4, 0.02 M EDTA, pH 7.4), a first wash at room temperature with 2×SSC containing 0.1% SDS and a second wash at 55° C. with 0.1×SSC containing 0.1% SDS, wherein said DNA encodes glucuronoxylomannan-O-acetylhydrolase;
- and
- (c) isolated DNA differing from the isolated DNAs of (a) and (b) above in codon sequence due to the degeneracy of the genetic code, and which encodes glucuronoxylomannan-O-acetylhydrolase.
1 more element
A recombinant glucuronoxylomannan-O-acetylhydrolase having an amino acid sequence shown in SEQ ID No. 31.
A kit containing purified glucuronoxylomannan-O-acetylhydrolase.
Dependencies are inferred from the claim text.