3-Hydroxy-3-methylglutaryl-CoA reductase polynucleotides in isoprenoid production
AssigneeRoche Vitamins Inc.
InventorsTatsuo Hoshino, Kazuyuki Ojima, Yutaka Setoguchi
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Abstract
The present invention relates to an isolated DNA sequence encoding an enzyme in the mevalonate pathway or the pathway from isopentenyl pyrophosphate to farnesyl pyrophosphate (i.e., HMG-CoA synthase, HMG-CoA reductase, mevalonate kinase, mevalonate pyrophosphate decarboxylase, and FPP synthase). Vectors and plasmids including such DNA, are also set forth. The invention also includes host cells transformed by such DNAs, or vectors or plasmids containing such DNAs. A process for the production of isoprenoids and carotenoids using such transformed host cells is also provided.
Claims15
An isolated DNA sequence comprising a DNA sequence that hybridizes to SEQ ID NO:2 under the following conditions:
- hybridization in 50% formnamide (v/v), 2% blocking agent, 5×SSC, 0.1% N-lauroylsarcosine (w/v), and 0.1% SDS at 42° C. overnight followed by two washes for 5 minutes each in 2×SSC and 0.1% SDS at room temperature followed by two additional washes of 15 minutes each in 0.1×SSC and 0.1% SDS at 68° C., wherein the DNA sequence encodes an amino acid sequence having 3-hydroxy-3-methylglutaryl-CoA reductase (HMG-CoA reductase) activity.
A process for the production of isoprenoids or carotenoids, which growing a host cell according to clam 10 under conditions wherein isoprenoids or carotenoids are produced.
An isolated DNA sequence comprising a DNA sequence that does not to SEQ ID NO:2 but which encodes an amino acid sequence that is identical to SEQ ID NO:7.
Dependencies are inferred from the claim text.