DNA vector for determining the presence of out-of-reading-frame mutations
AssigneeUniversity of South Florida
InventorsGary W. Litman, Noel A. Hawke, Robert N. Haire, Scott J. Strong
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Abstract
A DNA expression vector for positively selecting in-frame or out-of-reading-frame mutations in DNA sequences to be tested comprising a promotor operatively linked to an expressible reporter gene through a linkage sequence is disclosed. The linkage sequence includes at least two restriction sites and an engineered frameshift mutation. In an embodiment the frameshift is established by complementary sequences SEQ ID Nos:1 and 2. The expressible reporter gene is expressed as a fusion product including a green fluorescent protein and the promoter can be lacZ and inducible in E. coli.
Claims8
A DNA expression vector for positively selecting out-of-reading-frame mutations comprising a promotor operatively linked to an expressible reporter gene through a linkage sequence wherein said linkage sequence includes at least two restriction sites and an engineered frameshift mutation and wherein said expressible reporter gene when appropriately expressed provides a fusion product including a green fluorescent protein for positively selecting unknown out-of-reading-frame mutations.
A DNA expression vector for positively selecting out-of-reading-frame mutations comprising a promotor operatively linked to an expressible reporter gene through a linkage sequence wherein said linkage sequence includes at least two restriction sites and an engineered frameshift mutation which is established by complementary sequences SEQ ID Nos:
- 1 and 2 and wherein said expressible reporter gene when appropriately expressed provides a fusion product including a green fluorescent protein for positively selecting unknown out-of-reading-frame mutations.
Dependencies are inferred from the claim text.