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US 6,284,493 B1Granted
Method of synthesizing saccharide compositions
AssigneeNeose Technologies, Inc.
InventorStephen Roth
Life of this patent
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Abstract
A method of synthesizing saccharide compositions is described. In this method, an acceptor moiety is contacted with at least one donor saccharide in the presence of at least one cell surface-bound glycosyltransferase specific for catalyzing the coupling of the acceptor moiety with the donor saccharide. The acceptor moiety used is a carbohydrate, a protein, a lipid, or a glycolipid.
Claims28
A method, for the glycosyltransferase-catalyzed preparation of a preselected saccharide composition having a defined sequence of saccharide subunits by serially bonding saccharide units to an acceptor moiety comprising:
- (a) contacting said acceptor moiety with a first saccharide unit in the presence of a first cell surface genetically engineered to express a first cell surface-bound glycosyltransferase specific for catalyzing the linkage of said acceptor moiety with said first saccharide unit to form a first saccharide composition having a defined sequence of saccharide subunits;
- and
- (b) contacting said first saccharide composition with a second saccharide unit in the presence of a second cell surface genetically engineered to express a second cell surface-bound glycosyltransferase specific for catalyzing the linkage of said first saccharide composition with said second saccharide unit, wherein said second saccharide unit is different from said first saccharide unit, to form a preselected serially bound second saccharide composition having a defined sequence of saccharide subunits:
- wherein the first and second cell surface-bound glycosyltransferases are each selected from the group consisting of a galactosyltransferase, a sialyltransferase, a fucosyltransferase, an N-acetylgalactosaminyltransferase, an N-acetylglucosaminyltransferase, and a glucuronyltransferase.
1 more element
Dependencies are inferred from the claim text.