Asporogenous strain of bacillus subtilis and its use as a host for the preparation of heterologous products
AssigneeEniricerche S.p.A.
InventorsGianni Frascotti, Paola Cosmina, Guido Grandi
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Abstract
The invention relates to a new asporogenous strain of Bacillus subtilis which has been deposited at the Centraalbureau Voor Schimmelcultures under the number CBS 432.90. This strain, which shows a frequency of reversion to the formation of spores of less than about 10−8 and good plasmid stability, is suitable as a host in a host-vector system for the preparation of heterologous products of interest.
Claims7
A biologically pure culture of asporogenous strain Bacillus subtilis SMS275, suitable for use as a host component in a host-vector system, characterized in that said strain has a frequency of reversion to spore formers of less than 10−8 , plasmid stability, genetic markers leu, pyrD1, apr−, npr− and spoII:D−, and is deposited as CBS 432.90.
A process for the production of a heterologous polypeptide or protein or a precursor thereof, comprising the steps of:
- a) transforming an asporogenous strain Bacillus subtilis SMS275 with a plasmid vector containing a gene coding for a heterologous polypeptide or protein or a precursor thereof,
- wherein said asporogenous strain Bacillus subtilis SMS275 is characterized in that it has a frequency of reversion to spore formers of less than 10−8 , plasmid stability, genetic markers leu, pyrD1, apr−, npr− and spoII:D−, and is deposited as CBS 432.90;
- b) culturing the transformed asporogenous strain Bacillus subtilis of step (a) in a suitable culture medium containing a carbon source, a nitrogen source, mineral salts, leucine and uracil;
- and
- (c) recovering said heterologous polypeptide or protein or a precursor thereof.
Dependencies are inferred from the claim text.