Human oncogene induced secreted protein I
AssigneeHuman Genome Sciences, Inc.
InventorsHenrik S. Olsen, Steven M. Ruben
Life of this patent
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Abstract
The present invention relates to a novel protein, the Human Oncogene Induced Secreted Protein I (“HOIPS I”) protein. In particular, isolated nucleic acid molecules are provided encoding the human HOIPS I protein. HOIPS I polypeptides are also provided as are vectors, host cells and recombinant methods for producing the same. Also provided are diagnostic methods for detecting abnormal cell proliferation and differentiation disorders and therapeutic methods for treating the same.
Claims69
An isolated polynucleotide comprising a nucleic acid encoding amino acids 1 to 142 of SEQ ID NO:2.
An isolated polynucleotide comprising a nucleic acid encoding the mature polypeptide encoded by the cDNA clone contained in ATCC Deposit No. 97825.
An isolated polynucleotide comprising 24 contiguous nucleotides of the coding region of SEQ ID NO:1 or the complement thereof, wherein said isolated polynucleotide does not comprise any one of SEQ ID NOs:12-16.
An isolated polynucleotide, comprising a nucleic acid which encodes an amino acid sequence selected from the group consisting of:
- (a) amino acids −4 to 9 of SEQ ID NO:2, wherein said polynucleotide does not comprise SEQ ID NO:12;
- (b) amino acids 13 to 19 of SEQ ID NO:2,
- (c) amino acids 23 to 32 of SEQ ID NO:2, wherein said polynucleotide does not comprise SEQ ID NO:12 or SEQ ID NO:15;
- (d) amino acids 36 to 47 of SEQ ID NO:2, wherein said polynucleotide does not comprise SEQ ID NO:12, SEQ ID NO:15 or SEQ ID NO:16;
- (e) amino acids 54 to 63 of SEQ ID NO:2, wherein said polynucleotide does not comprise SEQ ID NO:12 or SEQ ID NO:16;
- (f) amino acids 90 to 100 of SEQ ID NO:2, wherein said polynucleotide does not comprise SEQ ID NO:12, SEQ ID NO:13 or SEQ ID NO:14;
- (g) amino acids 105 to 119 of SEQ ID NO:2, wherein said polynucleotide does not comprise SEQ ID NO:13 or SEQ ID NO:14;
- and
- (h) amino acids 125 to 132 of SEQ ID NO:2, wherein said polynucleotide does not comprise SEQ ID NO:12, SEQ ID NO:13 or SEQ ID NO:14.
An isolated polynucleotide comprising a first nucleic acid 95% or more identical to a reference nucleic acid encoding an amino acid sequence selected from the group consisting of:
- (a) amino acids −20 to 142 of SEQ ID NO:2;
- (b) amino acids −19 to 142 of SEQ ID NO:2;
- (c) amino acids 1 to 142 of SEQ ID NO:2;
- (d) the amino acid sequence of the mature polypeptide encoded by the cDNA clone contained in ATCC Deposit No. 97825;
- and
- (e) the amino acid sequence of the complete polypeptide encoded by the cDNA clone contained in ATCC Deposit No. 97825;
- wherein percent identity is calculated using Bestfit with the parameters set such that percentage of identity is calculated over the full length of the reference nucleic acid and that gaps in homology of up to 5% of the total number of nucleotides in the reference nucleic acid are allowed.
An isolated polynucleotide comprising a nucleic acid encoding an amino acid sequence, wherein, except for one to thirty conservative amino acid substitutions, said amino acid sequence is selected from the group consisting of:
- (a) amino acids −20 to 142 of SEQ ID NO:2;
- (b) amino acids −19 to 142 of SEQ ID NO:2;
- (c) amino acids 1 to 142 of SEQ ID NO:2;
- (d) the amino acid sequence of the mature polypeptide encoded by the cDNA clone contained in ATCC Deposit No. 97825;
- and
- (e) the amino acid sequence of the complete polypeptide encoded by the cDNA clone contained in ATCC Deposit No. 97825.
An isolated polynucleotide comprising a nucleic acid encoding an amino acid sequence 95% or more identical to a reference amino acid sequence selected from the group consisting of:
- (a) amino acids −20 to 142 of SEQ ID NO:2;
- (b) amino acids −19 to 142 of SEQ ID NO:2;
- (c) amino acids 1 to 142 of SEQ ID NO:2;
- (d) the amino acid sequence of the mature polypeptide encoded by the cDNA clone contained in ATCC Deposit No. 97825;
- and
- (e) the amino acid sequence of the complete polypeptide encoded by the cDNA clone contained in ATCC Deposit No. 97825;
- wherein percent identity is calculated using Bestfit with the parameters set such that percentage of identity is calculated over the full length of the reference amino acid sequence and that gaps in homology of up to 5% of the total number of amino acids in the reference amino acid sequence are allowed.
An isolated polynucleotide comprising a nucleic acid which is 95% or more identical to a reference nucleic acid, wherein said reference nucleic acid is selected from the group consisting of:
- (a) nucleotides 80 to 505 of SEQ ID NO:1;
- (b) nucleotides 23 to 505 of SEQ ID NO:1;
- and
- (c) nucleotides 20 to 505 of SEQ ID NO:1;
- wherein percent identity is calculated using Bestfit with the parameters set such that percentage of identity is calculated over the full length of the reference nucleic acid and that gaps in homology of up to 5% of the total number of nucleotides in the reference nucleic acid are allowed.
An isolated polynucleotide comprising a first nucleic acid which hybridizes (i) at 42° C. in a solution consisting of 50% formamide, 5×SSC, 50 mM sodium phosphate, 5×Denhardt's solution, 10% dextran sulfate, and 20 μg/ml denatured, sheared salmon sperm DNA;
- (ii) followed by washing in a solution consisting of 0.1×SSC at 65° C.;
- to a second nucleic acid having the nucleotide sequence of the coding region of SEQ ID NO:1 or the complement thereof;
- wherein said first nucleic acid is 15 or more nucleotides long and provided that said isolated polynucleotide does not comprise any one of SEQ ID NOs:12-16.
Dependencies are inferred from the claim text.