Process for the production of proteins in soluble form by modulation of an inducible promoter
AssigneeRoche Diagnostics GmbH
InventorsErhard Kopetzki, Günther Schumacher
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Abstract
The present invention provides a process for the expression in appropriate host cells of recombinant DNA which is under the control of an inducible promotor. In order to obtain the soluble form of recombinant protein, which would usually be obtained in insoluble form, the induction of the promotor is limited to less than 10% of the maximum induction in comparison with a standard system and the transcription rate of the DNA coding for the protein is correspondingly limited.
Claims30
A process for the expression of a protein in a transformed Escherichia coli host cell containing a DNA sequence encoding said protein and controlled by an inducible promoter, said process comprising limiting induction of said promoter to less than 10% of the maximum induction of said promoter thereby producing a greater amount of soluble and active forms of said protein than in the absence of the limited induction of said promoter.
Dependencies are inferred from the claim text.