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US 6,284,475 B1Granted
Assays for diagnosis of thrombophilic disease
AssigneeMount Sinai School of Medicine of the City of New York
InventorJacob H. Rand
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Abstract
The present invention provides methods for diagnosing and/or monitoring thrombophilic disease in a patient that can result from the antiphospholipid antibody syndrome (aPL syndrome). The methods of the invention are premised on the inhibition of binding of an anticoagulant protein, annexin, preferably annexin-V, to phospholipids by antiphospholipid (aPL) antibodies in a patient blood sample.
Claims22
A method of diagnosing and/or monitoring antiphospholipid antibody syndrome in a patient comprising
- a. incubating a phospholipid substrate with a test blood specimen suspected of containing antiphospholipid antibody from the patient in the presence of a known amount of an annexin for a time sufficient to allow the annexin to bind to the phospholipid substrate,
- b. removing unbound test specimen and annexin from the phospholipid substrate,
- c. measuring the amount of annexin bound to the phospholipid substrate in the presence of the test specimen, and
- d. comparing the amount of annexin bound in the presence of the test specimen to the same known amount of annexin bound to a phospholipid substrate in the presence of a control blood specimen, wherein a lower amount of annexin bound to the phospholipid substrate in the presence of the test specimen compared with the control specimen indicates the presence of antiphospholipid antibody syndrome in the individual.
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A method of diagnosing and/or monitoring a antiphospholipid antibody syndrome in a patient comprising
- a. incubating a phospholipid substrate with a test blood specimen suspected of containing antiphospholipid antibody from the patient in the presence of a known amount of an annexin for a time sufficient to allow the annexin to bind to the phospholipid substrate,
- b. removing unbound test specimen and annexin from the phospholipid substrate,
- c. measuring the amount of unbound annexin from step b, and
- d. comparing the amount of unbound annexin from step b to an amount of unbound annexin in a sample of annexin bound to a phospholipid substrate in the presence of a control blood specimen, wherein a higher amount of unbound annexin in the presence of the test specimen compared to the amount of unbound annexin in the presence of the control specimen indicates the presence of antiphospholipid antibody syndrome.
1 more element
Dependencies are inferred from the claim text.