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US 6,284,469 B1Granted
RNA export element and methods of use
AssigneeSalk Institute
InventorsThomas J. Hope, Romain Zufferey, Didier Trono, John Edward Donello
Life of this patent
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Abstract
A cis-acting posttranscriptional regulatory element (PRE) useful for efficient RNA export of RNA is provided. The element, termed WPRE, is originally derived from woodchuck hepatitis virus. The invention also provides a method for enhancing the expression of transgenes by insertion of the WPRE nucleic acid sequences in operably linkage with the transgene.
Claims6
A method for isolating at least one protein which binds to a nucleic acid sequence characterized as a cis-acting RNA export element, comprising:
- a) contacting a putative binding protein and the cis-acting RNA export element in vitro, wherein the c s-acting RNA export element comprises one or more sub-elements from Woodchuck hepatitis virus, selected from the group consisting of PREα, PREβ, and PREγ;
- and
- b) isolating the binding protein from the resulting complex of the RNA export element and the putative binding protein.
Dependencies are inferred from the claim text.