Diagnosis of penaeus monodon-type baculovirus by PCR
AssigneeAcademia Sinica
InventorsYa-Li Hsu, Todd Hsu
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Abstract
This invention relates to the methods of detecting Penaeus monodon baculovirus (MBV). Two methods are established: the first one is a polymerase chain reaction (PCR) and the second one is an ELISA. For the PCR method, two sets of primers are designed. The first set of primers is designed from the conserved sequences of nuclear polyhedrosis viruses (NPVs) DNA polymerase genes. The second set of primers is designed from the genomic DNA of MBV. The antibody for ELISA is an antiserum against the occlusion bodies of MBV.
Claims3
A method for detecting a Penaeus monodon baculovirus (MBV) comprising:
- ampliing DNA in a sample using a pair of primers consisting of the nucleic acid sequences of SEQ ID NO:3 and SEQ ID NO:4 by polymerase chain reaction (PCR) to obtain an amplified product;
- and
- analyzing said amplified product by electrophoresis.
A pair of MBV-specific primers consisting of DNA sequences of SEQ ID NO:3 and SEQ ID NO:4.
Dependencies are inferred from the claim text.