Variant of LAV viruses
AssigneeInstitut Pasteur
InventorsMarc Alizon, Pierre Sonigo, Simon Wain-Hobson, Luc Montagnier
Life of this patent
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Abstract
A variant of a LAV virus, designated LAVMAL and capable of causing AIDS. The cDNA and antigens of the LAVMAL virus can be used for the diagnosis of AIDS and pre-AIDS.
Claims3
A method for the detection of anti-LAV antibodies in a body fluid comprising:
- (A) immobilizing a predetermined amount of an antigenic peptide of LAVMAL, wherein the antigenic peptide is encoded by at least one cDNA fragment of LAVMAL produced by the process comprising treating a cDNA sequence that comprises a nucleotide sequence of FIGS. 7A-7I with at least one restriction enzyme, wherein the restriction enzyme is selected from the group consisting of AvaI, BamHI, BglII, EcoRI, HindIII, HincII, KpnI, NdeI, PstI, SacI, and XbaI;
- (B) contacting the immobilized LAVMAL peptide with a body fluid to be tested;
- (C) incubating the mixture of immobilized LAVMAL peptide and the body fluid;
- and
- (D) detecting the presence of anti-LAV antibody complexed to the immobilized LAVMAL peptide.
A method for the detection of anti-LAV antibodies in a body fluid comprising:
- (A) immobilizing a predetermined amount of an antigenic peptide of LAVMAL, wherein the antigenic peptide is encoded by is at least one cDNA fragment of LAVMAL produced by the process comprising treating a cDNA sequence that comprises a nucleotide sequence of FIGS. 7A-7I with at least one restriction enzyme, wherein the restriction enzyme is selected from the group consisting of AvaI, BamHI, BglII, EcoRI, HindIII, HincII, KpnI, NdeI, PstI, SacI, and XbaI;
- (B) contacting the immobilized LAVMAL peptides with a body fluid to be tested;
- (C) incubating the mixture of immobilized LAVMAL peptide and body fluid,
- (1) removing materials not complexed to the immobilized LAVMAL peptide,
- (2) adding labeled immunglobulins specifically reactive to said antibodies, and
- (3) detecting the presence of immobilized immunoglobulins/antibody complex;
- and
- (D) detecting the presence of anti-LAV antibody complexed to the immobilized LAVMAL peptide.
A diagnostic kit for the in vitro detection of antibodies against LAV comprising at least one antigenic LAVMAL peptide, a buffer solution, and labeled anti-human immunoglobulins, wherein the antigenic peptide is encoded by at least one CDNA fragment produced by the process comprising treating a cDNA sequence that comprises a nucleotide sequence of FIGS. 7A-7I with at least one restriction enzyme, wherein the restriction enzyme is selected from the group consisting of AvaI, BamHI, BglII, EcoRI, HindIII, HincII, KpnI, NdeI, PstI, SacI, and XbaI.
Dependencies are inferred from the claim text.