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AssigneesToyo Boseki Kabushiki Kaisha, Kazumori Funatsu
InventorsKazumori Funatsu, Hiroyuki Ijima, Kouji Nakazawa, Hiroshi Mizumoto
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The present invention provides a module for cell culture comprising a structure comprising hollow fibers and at least two spacers, wherein the spacers have small pores regularly arranged therein and the hollow fibers pass through the small pores and are arranged regularly at a very small distance, a hybrid artificial liver module wherein hepatocytes are immobilized in this module, and a method of cell culture wherein cells are immobilized in the lumen or extra-fiber space of the module of this module by the use of a centrifugal force and wherein the immobilized cells are cultured. The present invention also provides a method for cell culture wherein hepatocytes are cultured in a culture medium containing DMEM as a basal medium.
A module for cell culture comprising a structure comprising hollow fibers and at least two spacers, wherein the spacers have small pores regularly arranged therein and the hollow fibers pass through the small pores and are arranged regularly at a very small distance.
A method for preparing a cell culture comprising immobilizing cells in a lumen of a hollow fiber, or a lumen or extra-fiber space of the module in a module for cell culture, by the use of a centrifugal force, and culturing the immobilized cells.
A medium comprising a Dulbecco's modified Eagle's medium as a basal medium, and proline, epidermal growth factor, insulin, hydrocortisone, selenious acid, linoleic acid, copper sulfate and zinc sulfate.
A medium comprising a mixed medium of Dulbecco's modified Eagle's medium and a different medium as a basal medium, and proline, epidermal growth factor, insulin, hydrocortisone, selenious acid, linoleic acid, copper sulfate and zinc sulfate.
Dependencies are inferred from the claim text.