BHV-1 gene-deleted virus vaccine
AssigneeKansas State University Research Foundation
InventorShafiqul I. Chowdhury
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Abstract
A safe live recombinant virus as well as a vaccine is produced by the deletion of a portion of the native glycoprotein E (gE) coding region of bovine herpesvirus 1 (BHV-1) followed by the insertion of a plasmid including a foreign functional β-gal at the gE locus. The deletion of gE gene stably attenuates the virus and serves as an immunological marker differentiating vaccinated animals from infected animals. Moreover, production of β-gal allows easy assessment of gEΔ3.1IBRβ virus replication in vaccinated animals and serves as a phenotypic marker which distinguishes it from wild type virus replication in infected animals.
Claims30
An attenuated recombinant bovine herpesvirus type I virus comprising a bovine herpesvirus type I having a portion of a native coding region thereof deleted and replaced with a genetic insert, said portion being deleted only from the glycoprotein E coding region, said insert comprising the coding region of a foreign β-galactosidase gene and including human cytomegalovirus immediate early promoter, said recombinant virus expressing β-galactosidase in a host cell.
A recombinant virus having ATCC Accession No. VR-2637.
A plasmid having ATCC Accession No. 203607.
Dependencies are inferred from the claim text.