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US 6,284,220 B1Granted
Noninvasive imaging of nucleic acid vectors
AssigneeThe General Hospital Corporation
InventorsAlexei Bogdanov, Ching-Hsuan Tung, Ralph Weissleder
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Abstract
Nucleic acid-imaging compositions and methods for noninvasive imaging of a nucleic acid introduced into somatic tissues of an animal or human are disclosed. The noninvasive imaging enables quantitative assessment of the biodistribution of the introduced nucleic acid. The disclosed imaging compounds include a base-binding moiety, a phosphate-binding moiety, and a metal-binding moiety. A chelated metal is non-invasively detected for imaging by radioactivity or magnetic resonance.
Claims23
A nucleic acid-imaging compound comprising:
- a base binding moiety comprising a planar, polycyclic aromatic ring structure that intercalates between bases in double-stranded DNA, a phosphate binding moiety selected from the group consisting of polyamines, polyimines and a cationic polypeptide, a metal binding moiety selected from the group consisting of
- a mercaptoacetyl-triglycyl moiety;
- an N-acetyl-glycyl-cysteinyl(S-acetamidomethyl)-glycyl-cysteinyl(S-acetamidomethyl)-glycyl moiety;
- a glycyl-cysteinyl(S-acetamidomethyl)-glycyl-cysteinyl(S-acetamidomethyl)-glycyl moiety;
- a diethylenetriamine-pentaacetic acid (DTPA) moiety;
- an ethylenedicysteine moiety;
- a 1-imine-3-mercaptotutace moiety;
- a bis(aminoethanethiol)carboxylic acid moiety;
- a triethylenetetraamine-hexaacetic acid moiety;
- a ethylenediamine-tetraacetic acid (EDTA) moiety;
- a 1,2-diaminocyclohexane-N,N,N′,N′-tetraacetic acid moiety;
- a N,N′-di(2-hydroxybenzyl)ethylenediamine moiety;
- a N-(hydroxy-ethyl)ethylenediaminetriacetic acid moiety;
- a nitrilotriacetic acid moiety;
- a ethylene-bis(oxyethylene-nitrilo)tetraacetic acid moiety;
- a 1,4,7,10-tetraazacyclodo-decane-N,N′,N″,N′″-tetraacetic acid moiety;
- a 1,4,7,10-tetraaza-cyclododecane-N,N′,N″-triacetic acid moiety;
- a 1,4,7-tris(carboxymethvl)-10-(2′-hydroxypropyl)-1,4,7,10-tetraazocyclodecane moiety;
- a 1,4,7-triazacyclonane-N,N′,N″-triacetic acid moiety;
- and
- a 1,4,8,11-tetraazacyclotetra-decane-N,N′,N″,N′″-tetraacetic acid moiety;
- and
- a radioactive or paramagnetic metal or metal oxide bound by the metal-binding moiety.
20 more elements
Dependencies are inferred from the claim text.