Transgenic monocots plants with increased glycine-betaine content
AssigneeDekalb Genetics Corporation
InventorsThomas R. Adams, Paul C. Anderson, Richard J. Daines, William Gordon-Kamm, Albert P. Kausch, Michael T. Mann, Peter M. Orr, David C. Warner
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Abstract
The present invention provides a method for conferring tolerance or resistance to water or salt stress in a monocot plant, and/or altering the osmoprotectant content of a monocot plant, by introducing a preselected DNA segment into the plant. This invention also relates to the transformed cells and seeds, and to the fertile plants grown from the transformed cells and to their pollen.
Claims24
A transformed monocot plant, which plant is substantially tolerant or resistant to a reduction in water availability, the cells of which comprise a recombinant DNA segment comprising a preselected DNA segment encoding an enzyme which catalyzes the synthesis of glycine-betaine, wherein the enzyme is expressed in an amount effective to confer tolerance or resistance to the transformed plant to a reduction in water availability.
A fertile transgenic Zea mays plant comprising a recombinant DNA segment comprising a promoter operably linked to a first DNA segment encoding an enzyme which catalyzes the synthesis of glycine-betaine, wherein the first DNA segment is expressed so that the level of the enzyme is increased in the transgenic Zea mays plant, and wherein the recombinant DNA segment is heritable.
A method to increase water stress resistance or tolerance in monocot plant cells, comprising:
- (a) introducing into cells of a monocot plant an expression cassette comprising a preselected first DNA segment encoding an enzyme which catalyzes the synthesis of the glycine-betaine, operably linked to a promoter functional in the monocot plant cells, to yield transformed monocot plant cells;
- and
- (b) expressing the enzyme encoded by the preselected first DNA segment in the transformed monocot plant cells so as to render the transformed monocot plant cells substantially water stress tolerant or resistant.
A method to increase water stress resistance or tolerance in a monocot plant, comprising:
- (a) introducing into cells of a monocot plant an expression cassette comprising a preselected DNA segment encoding an enzyme which catalyzes the synthesis of the glycine-betaine, operably linked to a promoter functional in the monocot plant cells, to yield transformed monocot plant cells;
- and
- (b) regenerating a differentiated fertile plant from said transformed cells, wherein the enzyme encoded by the preselected DNA segment is expressed in cells of the plant so as to render the transformed monocot plant substantially water stress tolerant or resistant.
Dependencies are inferred from the claim text.