Rat ob-receptors and nucleotides encoding them
AssigneeMerck & Co., Inc.
InventorsJohn W. Hess, C. Thomas Caskey, Qingyun Liu, Michael Sean Phillips
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Abstract
The rat ob receptor gene has been isolated and cloned. Two different alleles have been identified: the wild-type, and the fa-allele which differs from the wild type by only one base pair. The base pair change, however introduces an MspI restriction site into the DNA sequence, and also results in an amino acid change. Also part of the invention are the receptors, vectors containing the nucleic acid encoding the receptors, host cells transformed with this gene, and assays which use the gene or protein and identify new ligands.
Claims16
An isolated nucleic acid encoding a rat ob-receptor (OB-R), said receptor comprising the sequence of SEQ ID NO:1.
An isolated DNA encoding fa OB-R said DNA comprising the sequence of SEQ ID NO:2 wherein nucleotide 880 is a C instead of an A.
An isolated nucleic acid encoding a rat fa ob-receptor (OB-R), said receptor comprising the sequence of SEQ ID NO:1 wherein amino acid 269 is a proline instead of a glutamine.
Dependencies are inferred from the claim text.