Parasite astacin metalloendopeptidase nucleic acid molecules and uses thereof
AssigneeHeska Corporation
InventorsCynthia Ann Tripp, Glenn Robert Frank, Robert B. Grieve
Life of this patent
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Abstract
The present invention relates to parasite astacin metalloendopeptidase proteins, nucleic acid molecules having sequences that encode such proteins, antibodies raised against such proteins and compounds that can inhibit the activities of parasite astacin metalloendopeptidases. The present invention also includes methods to obtain such nucleic acid molecules, proteins, antibodies and inhibitors. The present invention also includes therapeutic compositions comprising such nucleic acid molecules, proteins, antibodies and inhibitors as well as their use to protect animals from disease caused by parasites, such as heartworm infection.
Claims19
An isolated nucleic acid molecule selcctcd from the group consisting of
- (a) an isolated D. immitis nucleic aicd molecule selected om the group consisting of;
- (A) a D. immitis astacin metalloendopeptidase cDNA molecule and (B) a D. immitis astacin metallocndopeptidase mRNA molecule.
An isolated nucleic acid molecule, wherein said nucleic acid molecule cores a nucleic aid sequence selected from the group consisting of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:20,SEQ ID NO:21, SEQ ID NO:22, SEQ ID NO:29, SEQ ID NO:
- 30, SEQ ID NO:32, and SEQ ID NO:33.
A composition comprising an isolated parasite nucleic acid molecule selected from the group consisting of:
- a cDNA molecule and an mRNA molecule, wherein said nucleic acid molecule comprises a nucleic acid sequence selected from the group consisting of:
- (a) a nucleic acid sequence from the group consisting of:
- SEQ ID NO:
- 1, SEQ ID NO:2, SEQ ID NO:20, SEQ ID NO:21, SEQ ID NO:22, SEQ ID NO:29, SEQ ID NO:30, SEQ ID NO:32, and SEQ ID NO:33;
- (b) a nucleic acid sequence comprising at least 36 contiguous nucleotides of a nucleic acid sequence selected from the group consisting of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:20, SEQ ID NO:21, SEQ ID NO:22, SEQ ID NO:29, SEQ ID NO:30, SEQ ID NO:32, and SEQ ID NO:33, wherein said nucleic acid sequence comprises a nucleic acid sequence which encodes an extended zinc binding domain;
- and
- (c) a complement of a nucleic acid sequence of (a) or (b).
An isolated nucleic acid molecule selected from the group consisting of a cDNA molecule and an mRNA molecule, wherein said nucleic acid molecule comprises a nucleic acid sequence selected from the group consisting of:
- (a) a nucleic acid sequence selected from the group consisting of:
- SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:20, SEQ ID NO:21, SEQ ID NO:22, SEQ ID NO:29, SEQ ID NO:30, SEQ ID NO:32, and SEQ ID NO:33;
- (b) a nucleic acid sequence comprising at least 36 contiguous nucleotides of a nucleic acid sequence selected from the group consisting of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:20, SEQ ID NO:21, SEQ ID NO:22, SEQ ID NO:29, SEQ ID NO:30, SEQ ID NO:32, and SEQ ID NO:33, wherein said nucleic acid sequence comprises a nucleic acid sequence which encodes an extended zinc binding domain;
- and
- (c) a complement of a nucleic acid sequence of (a) or (b).
An isolated nucleic acid molecule selected from the group consisting of:
- (a) a nucleic acid molecule selected from the group consisting of:
- (A) a D. immitis astacin metalloendopeptidase cDNA molecule and (B) a D. immitis astacin metalloendopeptidase mRNA molecule, wherein said nucleic acid molecule of (A) or (B) has a nucleic acid sequence that hybridizes under conditions comprising (i) hybridizing in a solution consisting of 2×SSPE, 1% Sarkosyl, 5×Denhardts and 0.1 mg/ml denatured salmon sperm and (ii) washing in a solution consisting of 2×SSPE and 1% Sarkosyl, at 55° C.;
- with be complement of a nucleic acid sequence that encodes a protein having an amino acid sequence selected from the group consisting of:
- SEQ ID NO:4, SEQ ID NO:7, SEQ ID NO:31 and SEQ ID NO:34;
- and wherein said nucleic acid molecule encodes a protein having astacin metalloendopeptidase activity;
- and
- (b) a nucleic acid molecule comprising a nucleic acid sequence fully complementary to a nucleic acid sequence of (a).
A method to produce an astacin metalloendopeptidase protein, said method comprising culturing in an effective medium a recombinant cell expressing said protein, wherein said protein is encoded by a nucleic acid molecule selected from the group consisting of a cDNA molecule and an mRNA molecule, wherein said nucleic acid molecule comprises a nucleic acid sequence selected from the group consisting of:
- (a) a nucleic acid sequence selected from the group consisting of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:20, SEQ ID NO:21, SEQ ID NO:22, SEQ ID NO:29, SEQ ID NO:30, SEQ ID NO:32, and SEQ ID NO:33;
- and,
- (b) a nucleic acid sequence comprising at least 36 contiguous nucleotides of a nucleic acid sequence selected from the group consisting of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:20, SEQ ID NO:21, SEQ ID NO:22, SEQ ID NO:29, SEQ ID NO:30, SEQ ID NO:32, and SEQ ID NO:33, wherein said nucleic acid sequence, comprises a nucleic acid sequence which encodes an extended zinc binding domain.
Dependencies are inferred from the claim text.