Methods for conversion of protein isoforms
AssigneeSchering Corporation
InventorsSusan Cannon-Carlson, Andres Frei, Seoju Lee, Roland Mengisen, Marcio Voloch, David C. Wylie
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Abstract
The present invention provides methods for isolating adjunct isoforms of proteins and converting them to the desired protein. In preferred embodiments, the present invention contemplates the use of acid solutions or zinc solutions to cleave a chemical group from a desired protein. In further preferred embodiments, the present invention contemplates the oxidation of reduced sulfhydryl groups with cleavage of chemical groups to form a functional desired protein.
Claims17
A method for increasing the yield of an interferon alpha composition, comprising converting a pyruvate adjunct isoform of interferon alpha into interferon alpha by exposing said pyruvate adjunct isoform of interferon alpha to a solution having a pH of at least 5.0.
Dependencies are inferred from the claim text.