Hedgehog-derived polypeptides
AssigneeThe Johns Hopkins University School of Medicine
InventorsPhilip A. Beachy, Randall T. Moon
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Abstract
The present invention provides two novel polypeptides, referred to as the “N” and “C” fragments of hedgehog, or N-terminal and C-terminal fragments, respectively, which are derived after specific cleavage at a G↓CF site recognized by the autoproteolytic domain in the native protein. Also provided are methods of use of the N and C fragments.
Claims4
A substantially pure N-terminal fragment of a vertebrate hedgehog polypeptide, which fragment is produced by specific cleavage of a Gly↓Cys Phe cleavage site and wherein the fragment has a molecular weight of about 19 kD by non-reducing SDS-PAGE.
A substantially pure C-terminal fragment of a vertebrate hedgehog polypeptide, which fragment is produced by specific cleavage of a Gly↓Cys Phe cleavage site and wherein the fragment has a molecular weight of about 25 kD by non-reducing SDS-PAGE.
Dependencies are inferred from the claim text.