Methods and compositions for peptide synthesis
AssigneeTrimeris, Inc.
InventorsMyung-Chol Kang, Brian Bray, Maynard Lichty, Catherine Mader
Life of this patent
Estimating the term…
Abstract
The present invention relates, first, to methods for the synthesis of peptides, in particular T-20 (also referred to as “DP-178”; SEQ ID NO:1) and T-20-like peptides. Such methods utilize solid and liquid phase synthesis procedures to synthesize and combine groups of specific peptide fragments to yield the peptide of interest. The present invention further relates to individual peptide fragments which act as intermediates in the synthesis of the peptides of interest (e.g., T-20). The present invention still further relates to groups of such peptide intermediate fragments which can be utilized together to produce full length T-20 and T-20-like peptides.
Claims34
A set of peptide fragments comprising a set selected from the group consisting of:
- (a) YTSLIHSLIEESQNQQ (SEQ ID NO:4), EKNEQELLELDKWASLWNWF (SEQ ID NO:12);
- (b) YTSLIHSLIEESQNQQ (SEQ ID NO:4), EKNEQELLEL (SEQ ID NO:11), DKWASLWNWF (SEQ ID NO:18);
- (c) YTSLIHSLIEESQNQQ (SEQ ID NO:4), EKNEQELLEL (SEQ ID NO:11), DKWASLWNW (SEQ ID NO:17);
- (d) YTSLIHSL (SEQ ID NO:2), IEESQNQ (SEQ ID NO:6), EKNEQELLELDKWASLWNWF (SEQ ID NO:12);
- (e) YTSLIHSL (SEQ ID NO:2), IEESQNQ (SEQ ID NO:6), EKNEQELLEL (SEQ ID NO:11), DKWASLWNWF (SEQ ID NO:18);
- (f) YTSLIHSL (SEQ ID NO:2), IEESQNQ (SEQ ID NO:6), EKNEQELLEL (SEQ ID NO:11), DKWASLWNW (SEQ ID NO:17);
- (g) YTSLIHSL (SEQ ID NO:2), IEESQNQQ (SEQ ID NO:7), EKNEQELLELDKWASLWNWF (SEQ ID NO:12);
- (h) YTSLIHSL (SEQ ID NO:2), IEESQNQQ (SEQ ID NO:7), EKNEQELLEL (SEQ ID NO:11), DKWASLWNWF (SEQ ID NO:18);
- (i) YTSLIHSL (SEQ ID NO:2), IEESQNQQ (SEQ ID NO:7), EKNEQELLEL (SEQ ID NO:11), DKWASLWNW (SEQ ID NO:17);
- (j) YTSLIHSLIEESQNQQ (SEQ ID NO:4), EKNEQEL (SEQ ID NO:10), LELDKWASLWNWF (SEQ ID NO:16);
- (k) YTSLIHSLIEESQNQQ (SEQ ID NO:4), EKNEQEL (SEQ ID NO:10), LELDKWASLWNW (SEQ ID NO:15);
- (l) YTSLIHSL (SEQ ID NO:2), IEESQNQ (SEQ ID NO:6), EKNEQEL (SEQ ID NO:10), LELDKWASLWNWF (SEQ ID NO:16);
- (m) YTSLIHSL (SEQ ID NO:2), IEESQNQ (SEQ ID NO:6), EKNEQEL (SEQ ID NO:10), LELDKWASLWNW (SEQ ID NO:15);
- (n) YTSLIHSL (SEQ ID NO:2), IEESQNQQ (SEQ ID NO:7), EKNEQEL (SEQ ID NO:10), LELDKWASLWNWF (SEQ ID NO:16);
- (o) YTSLIHSL (SEQ ID NO:2), IEESQNQQ (SEQ ID NO:7), EKNEQEL (SEQ ID NO:10), LELDKWASLWNW (SEQ ID NO:15),
- (p) YTSLIHSLIEESQNQ (SEQ ID NO:3), QEKNEQELLELDKWASLWNW (SEQ ID NO:8);
- (q) YTSLIHSLIEESQNQ (SEQ ID NO:3), QEKNEQELLELDKWASLWNWF (SEQ ID NO:9);
- (r) YTSLIHSLIEESQNQQEK (SEQ ID NO:5), NEQELLELDKWASLWNWF (SEQ ID NO:14);
- (s) YTSLIHSLIEESQNQQEK (SEQ ID NO:5), NEQELLELDKWASLWNW (SEQ ID NO:13);
- and
- (t) YTSLIHSLIEESQNQQ (SEQ ID NO:4), EKNEQELLELDKWASLWNW (SEQ ID NO:19).
A peptide selected from the group consisting of:
- YTSLIHSL (SEQ ID NO:2), IEESQNQ (SEQ ID NO:6), IEESQNQQ (SEQ ID NO:7), QEKNEQELLELDKWASLWNW (SEQ ID NO:8), EKNEQEL (SEQ ID NO:10), EKNEQELLEL (SEQ ID NO:11), NEQELLELDKWASLWNW (SEQ ID NO:13), LELDKWASLWNW (SEQ ID NO:15), LELDKWASLWNWF (SEQ ID NO:16), DKWASLWNW (SEQ ID NO:17), DKWASLWNWF (SEQ ID NO:18), and
- EKNEQELLELDKWASLWNW (SEQ ID NO:19).
Dependencies are inferred from the claim text.