Use of derivatized reagents in chemiluminescence and electrochemiluminescence detection methods
AssigneeRoche Diagnostics GmbH
InventorsMartin Egger, Hans-Peter Josel, Gabriele Punzmann
Life of this patent
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Abstract
The invention concerns methods for the detection of an analyte in a sample by chemiluminescence or electrochemiluminescence using derivatized test reagents. In addition new reagents and reagent kits for chemiluminescence and electrochemiluminescence detection methods are disclosed.
Claims20
A method for the detection of an analyte in a sample by chemiluminescence or electrochemiluminescence in which the sample is contacted with a test reagent comprising at least one chemiluminescent or electrochemiluminescent labelling group coupled to an analyte-specific receptor and other test components, wherein the labelling group is selected from the group consisting of metal complexes having a structure of the general formula Mn(L1L2L3)m in which M is a divalent or trivalent metal cation selected from rare earth and transition metal cations, L1, L2 and L3 are the same or different and are ligands with at least 2 heterocycles containing nitrogen, and n and m are each independently of one another integers of 1 to 10, wherein the oxidizable chemical groups on the labelled receptor and optionally on other test components are protected by derivatization, and a signal from the labelling group is detected as a measure of the analyte in the sample.
A reagent kit for the detection of an analyte, the kit comprising:
- (a) an analyte-specific receptor which carries at least one chemiluminescence or electrochemiluminescence labelling group, wherein the labelling group is selected from the group consisting, of metal complexes having a structure of the cyeneral formula Mn(L1L2L3)m in which M is a divalent or trivalent metal cation selected from rare earth and transition metal cations, L1, L2 and L3 are the same or different and are ligands with at least 2 heterocycles containing nitrogen, and n and m are each independently of one another integers of 1 to 10 and
- (b) additional test components, wherein oxidizable chemical groups on the labelled receptor and optionally on additional test components are protected by derivatization.
A conjugate of a biological substance and at least one chemiluminescent or electrochemiluminescent labelling group, wherein the labelling group is selected from the group consisting of metal complexes having a structure of the general formula Mn(L1L2L3)m in which M is a divalent or trivalent metal cation selected from rare earth and transition metal cations, L1, L2 and L3 are the same or different and are ligands with at least 2 heterocycles containing nitrogen, and n and in are each independently of one another integers of 1 to 10, wherein the oxidizable chemical groups on the biological substance are protected by derivatization.
Dependencies are inferred from the claim text.