Device for non-pulsating post-column derivatization
OwnerJürgen Werringloerinventor
Life of this patent
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Abstract
A device for performing a post-column derivatization in connection with liquid chromatography includes a separating column, a detector, an eluent withdrawal unit, connected to an outlet of the separating column, that withdraws eluents from the separating column, a reagent supply unit, arranged between the outlet of the separating column and an inlet of the detector, that supplies reagents to the eluents withdrawn from the separating column and a pump that simultaneously pumps eluents from the eluent withdrawal unit and pumps reagents to the reagent supply unit, wherein the pumping of reagents takes place free of pulsations.
Claims16
A device for performing a post-column derivatization in connection with liquid chromatography, comprising:
- a separating column;
- a detector;
- an eluent withdrawal unit, connected to an outlet of the separating column, that withdraws eluents from the separating column;
- a reagent supply unit, arranged between the outlet of the separating column and an inlet of the detector, that supplies reagents to the eluents withdrawn from the separating column;
- a pump that simultaneously pumps eluents from the eluent withdrawal unit and pumps reagents to the reagent supply unit, wherein the pumping of reagents takes place free of pulsations;
- at least one eluent syringe, connected to the eluent withdrawal unit and the pump by a withdrawal tube, that collects eluents pumped from the eluent withdrawal unit;
- and
- at least one reagent syringe, connected to the reagent supply unit and the pump by a supply tube, that supplies reagents pumped to the reagent supply unit, wherein the at least one eluent syringe and the at least one reagent syringe operate oppositely to one another and during pumping of reagents from the at least one reagent syringe to the reagent supply unit, the at least on eluent syringe is passively filled with eluents from the eluent withdrawal unit.
A method of performing post-column derivatization in connection with liquid chromatography, comprising:
- separating eluents in a separating column;
- withdrawing the eluents from the separating column to an eluent withdrawal unit;
- supplying reagents from a reagent supplying unit to the eluents withdrawn from the separating column;
- simultaneously pumping eluents from the eluent withdrawal unit and pumping reagents to the reagent supply unit;
- collecting the eluents pumped from the eluent withdrawal unit in at least one eluent syringe;
- and
- passively filling the at least one eluent syringe with eluents from the eluent withdrawal unit during pumping of reagents from at least one reagent syringe to the reagent supply unit.
Dependencies are inferred from the claim text.