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AssigneeWashington State University Research Foundation
InventorsRodney B. Croteau, Bernd M. Lange
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The present invention relates to isolated DNA sequences which code for the expression of plant 1-deoxy-D-xylulose-5-phosphate reductoisomerase protein, such as the sequence presented in SEQ ID NO:1 which encodes a 1-deoxy-D-xylulose-5-phosphate reductoisomerase protein from peppermint (Mentha x piperita). Additionally, the present invention relates to isolated plant 1-deoxy-D-xylulose-5-phosphate reductoisomerase protein. In other aspects, the present invention is directed to replicable recombinant cloning vehicles comprising a nucleic acid sequence which codes for a plant 1-deoxy-D-xylulose-5-phosphate reductoisomerase, to modified host cells transformed, transfected, infected and/or injected with a recombinant cloning vehicle and/or DNA sequence of the invention.
An isolated nucleic acid molecule that hybridizes under stringent conditions to the nucleic acid molecule of SEQ ID NO:1, or to the complement of the nucleic acid molecule of SEQ ID NO:1, provided that said isolated nucleic acid molecule does not consist of a nucleic acid sequence selected from the group consisting of SEQ ID NO:10 and SEQ ID NO:11 or a nucleic acid sequence complementary to a nucleic acid sequence selected from the group consisting of SEQ ID NO:10 and SEQ ID NO:11, wherein said stringent hybridization conditions consist of hybridization in 3×SSC at 65° C. for 16 hours, followed by two washes in 2×SSC at 23° C. for 20 minutes per wash, followed by one wash in 0.5×SSC at 55° C. for 30 minutes.
A replicable vector comprising a first nucleic acid molecule that hybridizes under stringent conditions to a second nucleic acid molecule consisting of the nucleic acid sequence set forth in SEQ ID NO:1, or to a third nucleic acid molecule consisting of the complement of the nucleic acid sequence set forth in SEQ ID NO:1, provided that said first nucleic acid molecule does not consist of a nucleic acid sequence selected from the group consisting of SEQ ID NO:10 and SEQ ID NO:11 or a nucleic acid sequence complementary to a nucleic acid sequence selected from the group consisting of SEQ ID NO:10 and SEQ ID NO:11, wherein said stringent hybridization conditions consist of hybridization in 3×SSC at 65° C. for 16 hours, followed by two washes in 2×SSC at 23° C. for 20 minutes per wash, followed by one wash in 0.5×SSC at 55° C. for 30 minutes.
A method of enhancing the level of expression of 1-deoxy-D-xylulose-5-phosphate reductoisomerase protein in a host cell comprising introducing into said host cell a replicable expression vector comprising a nucleic acid molecule that encodes a 1-deoxy-D-xylulose-5-phosphate reductoisomerase protein under conditions that enable expression of said protein in said host cell, wherein said nucleic acid molecule hybridizes to the complement of a nucleic acid molecule consisting of the nucleic acid sequence set forth in SEQ ID NO:1 under hybridization conditions consisting of hybridization in 3×SSC at 65° C. for 16 hours, followed by two washes in 2×SSC at 23° C. for 20 minutes per wash, followed by one wash in 0.5×SSC at 55° C. for 30 minutes.
A method of reducing the level of expression of 1-deoxy-D-xylulose-5-phosphate reductoisomerase protein in a host cell comprising introducing into said host cell a replicable expression vector comprising a nucleic acid molecule that expresses an RNA molecule that hybridizes under stringent conditions to the nucleic acid sequence of SEQ ID NO:1, wherein said stringent hybridization conditions consist of hybridization in 3×SSC at 65° C. for 16 hours, followed by two washes in 2×SSC at 23° C. for 20 minutes per wash, followed by one wash in 0.5×SSC at 55° C. for 30 minutes.
Dependencies are inferred from the claim text.