Sorangium polyketide synthases and encoding DNA therefor

A purified and isolated DNA molecule that comprises a polyketide synthase open reading frame, wherein said open reading frame encodes one or more modules, and wherein one or more domains thereof are selected from the groups consisting of

• (a) a ketosynthase domain of SEQ ID NO:1 (FIG. 3) identified, as amino acid positions, 1 to 339, 1698 to 2121, and 3240-3665 thereof;
• (b) a ketosynthase domain of SEQ ID NO:2 (FIG. 4) identified, as amino acid positions, 34 to 457, 1812 to 2235, 3356-3781, and 5134-5558 thereof;
• (c) an acyltransferase domain of SEQ ID NO:1 (FIG. 3) identified, as amino acid positions, 450 to 780, 2232-2564, and 3775-4107 thereof;
• (d) an acyltransferase domain of SEQ ID NO:2 (FIG. 4) identified, as amino acid positions, 568 to 895, 2341 to 2673, 3887-4219, and 5664-5991 thereof;
• (e) a dehydratase domain of SEQ ID NO:1 (FIG. 3) identified, as amino acid positions, 807 to 985, and 4134 to 4312 thereof;
• (f) a dehydratase domain of SEQ ID NO:2 (FIG. 4) identified, as amino acid positions, 922 to 1100, 4246 to 4424, and 6018 to 6095 thereof;
• (g) a ketoreductase domain of SEQ ID NO:1 (FIG. 3) identified, as amino acid positions, 1315 to 1500, 2861 to 3045, and 4641 to 4825 thereof;
• (h) a ketoreductase domain of SEQ ID NO:2 (FIG. 4) identified, as amino acid positions, 1429 it 1614, 2978 to 3162, and 4754 to 4939 thereof;
• (i) an acyl carrier protein domain of SEQ ID NO:1 (FIG. 3 ) identified, as amino acid positions, 1592 to 1675, 3134 to 3218, and 4918 to 5001 thereof;
• and
• (j) an acyl carrier protein domain of SEQ ID NO:2 (FIG. 4) identified, as amino acid positions, 1706 to 1789, 3250 to 3333, and 5031 to 5114 thereof.