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US 6,280,995 B1Granted
Pectate lyases
AssigneeNovozymes A/S
InventorsLene Nonboe Andersen, Martin Schülein, Niels Erik Krebs Lange
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Abstract
The present invention relate to microbial pectate lyase, more specifically to microbial enzyme exhibiting pectate lyase activity as their major enzymatic activity in neutral and alkaline pH ranges, to a method of producing such an enzyme, and a method for using such enzymes in the textile, detergent, and cellulose fiber processing industries.
Claims5
An isolated polynucleotide molecule encoding a polypeptide having pectate lyase activity selected from the group consisting of:
- a) polynucleotide molecules comprising a nucleotide sequence as shown in SEQ ID NO:
- 1 from nucleotide 1 to nucleotide 1026;
- b) polynucleotide molecules that encode a polypeptide having at least 95% identity to the amino acid sequence of SEQ ID NO:
- 2 from amino acid residue 1 to amino acid residue 341, wherein said identity is determined by the GAP program, using a GAP creation peialty of 3.0 and a GAP extension penalty of 0.1;
- and
- c) polynucleotide molecules encoding a pectate lyase, wherein said polynucleotide molecules hybridize to the DNA sequence of SEQ ID NO:
- 1 under high stringency conditions, wherein said high stringency conditions comprise hybridization in 5×SSC at 45° C. and washing in 2×SSC at 70° C.
4 more elements
Dependencies are inferred from the claim text.