Type II restriction endonuclease, Hpy99I, obtainable from Helicobacter pylori J99 and a process for producing the same
AssigneesNew England Biolabs, Inc., Vanderbilt University
InventorsRichard D. Morgan, Qing Xu
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Abstract
In accordance with the present invention, there is provided a novel restriction endonuclease and its DNA obtainable from Helicobacter pylori J99 (NEB#1237), hereinafter referred to as “Hpy99I”, which endonuclease: (1) recognizes the nucleotide sequence 5′-CGWCG-3′ in a double-stranded DNA (wherein G represents guanine, C represents cytosine, A represents adenine, T represents thymine and W represents either A, or T); (2) cleaves double-stranded PhiX174 DNA to produce 8 fragments, including fragments of 3063, 629, 602, 447, 389, and 176 base pairs, and 2 fragments smaller than 100 base pairs.
Claims3
A substantially pure Type II restriction endonuclease obtainable from Helicobacter pylori (ATCC Patent Accession No. PTA-2865) recognizing the following base sequence in double-stranded deoxyribonucleic acid molecules:
- 5′-CGWCG-3′
- 3′-GCWGC-5′.
Dependencies are inferred from the claim text.