Sialyltransferases
OwnerDmitri Kapitonov, Robert K. Yuinventors
Life of this patent
Estimating the term…
Abstract
The present invention relates to isolated sialyltransferases, such as human or mouse GM3 synthase, human or mouse 4ST3GalVI, or human 7STGalNAcV sialyltransferase polypeptide, biologically-active polypeptide fragments thereof, and nucleic acids which code for it. This polypeptide has various activities including sialyltransferase activity. The invention relates to all aspects of sialyltransferase, or homologs thereof, including assays for modulators, activators, ligands, etc. The invention also relates to sialyltransferases expressed in cells and methods of using such cells to engineer specific sugar chains.
Claims13
An isolated nucleic acid, comprising a nucleotide sequence coding for a full-length mammalian GM3-synthase, or, coding for a biologically-active polypeptide fragment thereof, with the proviso that said nucleic acid is not AA604937-1998;
- AA833733-1998;
- AA848052-1998;
- AA648992-1997;
- AA648973-1997;
- AI150920-1998;
- N40410-1996;
- AA969297-1998;
- AA429546-1997;
- AA482611-1997;
- AI038475-1998;
- AA386324-1997;
- AI359060-1999;
- AA448834-1997;
- AA827206-1998;
- AA297799-1997;
- W56658-1996;
- AA649318-1997;
- W56550-1996;
- AA383443-1997;
- AI364968-1999;
- AA428458-1997;
- AA954095-1998;
- R93279-1996;
- T95985-1995;
- R13431-1995;
- AI123263-1998;
- AI089834-1998;
- R40943-1995;
- AA383409-1997;
- AA298186-1997;
- N98461-1996;
- AA928319-1998;
- AA359739-1997;
- AA934042-1998;
- AA127477-1996;
- C01821-1996;
- R93185-1996;
- W30980-1996;
- R88774-1995;
- AI192267-1998;
- R18785-1995;
- AA757529-1998;
- T95887-1995;
- T48553-1995;
- R88773-1995;
- AA448833-1997;
- AA395997-1997;
- AA208995-1997;
- AA274576-1997;
- Ai322283-1998;
- AA117276-1996;
- W36875-1996;
- Ai324739-1998;
- AA068897-1997;
- AA638055-1997;
- AA592148-1997;
- AA038269-1996;
- AA656084-1997, these numbers being accession numbers and their dates.
An isolated nucleic acid, comprising a nucleotide sequence coding for a full-length mammalian GM3-synthase, or, coding for a biologically-active polypeptide fragment thereof, and which hybridizes under high stringency conditions to the human or mouse nucleotide sequence set forth in FIG. 1 (SEQ ID NO:3), or a complement thereto, and which has at least 95% sequence identity to said nucleotide sequences, or its complement,
- with the proviso that said nucleic acid is not AA604937-1998;
- AA833733-1998;
- AA848052-1998;
- AA648992-1997;
- AA648973-1997;
- AI150920-1998;
- N40410-1996;
- AA969297-1998;
- AA429546-1997;
- AA482611-1997;
- AI038475-1998;
- AA386324-1997;
- AI359060-1999;
- AA448834-1997;
- AA827206-1998;
- AA297799-1997;
- W56658-1996;
- AA649318-1997;
- W56550-1996;
- AA383443-1997;
- AI364968-1999;
- AA428458-1997;
- AA954095-1998;
- R93279-1996;
- T95985-1995;
- R13431-1995;
- AI123263-1998;
- AI089834-1998;
- R40943-1995;
- AA383409-1997;
- AA298186-1997;
- N98461-1996;
- AA928319-1998;
- AA359739-1997;
- AA934042-1998;
- AA127477-1996;
- C01821-1996;
- R93185-1996;
- W30980-1996;
- R88774-1995;
- AI192267-1998;
- R18785-1995;
- AA757529-1998;
- T95887-1995;
- T48553-1995;
- R88773-1995;
- AA448833-1997;
- AA395997-1997;
- AA208995-1997;
- AA274576-1997;
- Ai322283-1998;
- AA117276-1996;
- W36875-1996;
- Ai324739-1998;
- AA068897-1997;
- AA638055-1997;
- AA592148-1997;
- AA038269-1996;
- AA656084-1997, these numbers being accession numbers and their dates.
An isolated nucleic acid consisting essentially of any continuous sequence of 12-100 base pairs, or a complement thereto, selected from the nucleotide sequence set forth 30 in FIG. 1 (SEQ ID NO:3).
Dependencies are inferred from the claim text.