Human PEC-60-like protein and DNA encoding the same
AssigneeSagami Chemical Research Center
InventorsSeishi Kato, Tomoko Yamaguchi, Shingo Sekine, Kouju Kamata
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Abstract
The present invention provides a human PEC-60-like protein, a gastrointestinal hormone excreted by a stomach tissue and a cDNA encoding this protein. The protein and the gene of the present invention can provide a protein containing the amino acid sequence represented by Sequence No. 1 and a DNA encoding said protein exemplified as a cDNA containing the base sequence represented by Sequence No. 1. as well as a human cDNA encoding a human PEC-60-like protein and said protein by the expression of this human cDNA recombinant.
Claims17
An isolated polynucleotide selected from the group consisting of:
- (a) a polynucleotide comprising the nucleotide sequence of SEQ ID NO:1;
- (b) a polynucleotide comprising the nucleotide sequence of SEQ ID NO:2;
- (c) a polynucleotide comprising the nucleotide sequence of SEQ ID NO:2 from nucleotide 43 to nucleotide 303;
- (d) a polynucleotide encoding a protein comprising the amino acid sequence of SEQ ID NO:3;
- (e) a polynucleotide encoding a protein comprising the amino acid sequence of SEQ ID NO:3 from amino acid 27 to amino acid 86;
- (f) a polynucleotide encoding a protein comprising a fragment of the amino acid sequence of SEQ ID NO:3, the fragment comprising the amino acid sequence of SEQ ID NO:3 from amino acid 27 to amino acid 86;
- (g) a polynucleotide encoding a protein comprising a fragment of the amino acid sequence of SEQ ID NO:3, the fragment having an activity of inhibiting glucose-induced insulin secretion;
- and
- (h) a polynucleotide which is a polymorphic variant of a polynucleotide of (a)-(g) above.
An isolated protein, wherein said protein comprises an amino acid sequence selected from the group consisting of:
- (a) the amino acid sequence of SEQ ID NO:3;
- (b ) the amino acid sequence of SEQ ID NO:3 from amino acid 27 to amino acid 86;
- (c) a fragment of the amino acid sequence of SEQ ID NO:3, the fragment comprising the amino acid sequence of SEQ ID NO:3 from amino acid 27 to amino acid 86;
- and
- (d) a fragment of the amino acid sequence of SEQ ID NO:3, the fragment having an activity of inhibiting glucose-induced insulin secretion;
- the protein being substantially free from other human proteins.
Dependencies are inferred from the claim text.