Whole blood/mitogen assay for the early detection of a subject with cancer and kit
AssigneeYoreh Biotechnologies, Ltd.
InventorTamar Jehuda Cohen
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Abstract
This invention provides a method of detecting a subject having ovarian or breast cancer, comprising the following steps; a) obtaining a whole blood sample from the subject; b) incubating the whole blood sample in a culture in the presence of a media containing a mitogen, so as to induce polyclonal activation of lymphocytic cells to produce tumor antigen associated antibodies; c) exposing the resultant culture of step b) to an specific tumor antigen, thereby allowing an antigen-antibody immune complex to form; d) detecting the antigen-antibody immune complex of step c); wherein the presence of tumor antigen associated antibody is indicative of the subject having the cancer.
Claims18
A method for the detection of tumor antigen associated antibodies in a sample obtained from a subject, comprising the following steps:
- a) obtaining a whole blood sample from the subject;
- b) incubating the whole blood sample in a culture in the presence of a mitogen containing media, so as to induce polyclonal activation of lymphocytic cells to produce to specific tumor antigen associated antibodies;
- c) exposing the resultant culture of step b) to a tumor antigen, thereby allowing an antigen-antibody immune complex to form;
- and
- d) detecting the presence of an antigen-antibody immune complex of step c);
- wherein the presence of antigen specific antibodies in indicative of the subject being with a tumor.
A method for screening a subject having cancer, comprising the following steps:
- a) obtaining a whole blood sample from the subject;
- b) incubating the whole blood sample in a culture in the presence of a mitogen containing media, so as to induce polyclonal activation of lymphocytic cells to produce specific tumor antigen associated antibodies;
- c) exposing the culture of step b) to an tumor antigen, thereby allowing an antigen-antibody immune complex to form;
- and
- d) detecting the presence of antigen-antibody immune complex of step c);
- wherein the presence of tumor antigen specific antibodies is indicative of the having cancer.
A kit for the detection of specific tumor antigen associated antibody from a subject, comprising:
- a container for collecting whole blood samples, wherein the container contains a media containing mitogen, effective to induce polyclonal activation of lymphocytic cells leading to antibody production and an assay for the detection of the specific tumor antigen associated antibody.
Dependencies are inferred from the claim text.