Use of tyramide coating and physical separation for isolating cells or particles of interest
AssigneeVerve, Ltd.
InventorDavid R. Kaplan
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Abstract
The present invention relates to methods of tyramide coating cells or particles for physical separation, using catalyzed reporter deposition and serial amplification staining. A catalyzed reporter deposition or an analyte dependent enzyme activation system is described for detecting and/or quantitating an analyte of interest on the surface of a cell by physical separation. Also described is a method for serial amplification staining by tyramide coating cells or particles which possess an analyte of interest or a solid phase to which an analyte is bound.
Claims20
A method of isolating cells or particles from a sample, wherein the cells or particles have an analyte of interest on the surface of said cells or particles, comprising:
- exposing particles or unfixed cells to a binding partner specific for the analyte of interest and a labeling molecule comprising tyramide, such that the bound cells or particles are coated with tyramide, and isolating said tyramide coated cells or particles with a physical separation device.
Dependencies are inferred from the claim text.