Rh blood group antigen compositions and methods of use
AssigneesBoard of Regents, The University of Texas Systems, Baylor College of Medicine
InventorsL. Scott Rodkey, Marwan A. Yared, Kenneth J. Moise, Jr.
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Abstract
Disclosed are novel protein and peptide compositions comprising soluble and bound forms of immunologically-active blood group antigens including mammalian Rh antigens. In preferred embodiments methods for the isolation and purification of serologically-active human Rh antigens such as D, c, C, E, and e are disclosed. Also disclosed are methods for the adsorption of immunologically-active Rh antigens to solid supports. Diagnostic kits, methods, and devices for the detection of Rh antibodies in clinical and non-clinical samples are also disclosed. Devices, compositions and methods for the isolation, purification and quantitation of anti-Rh antibodies from solution are also provided.
Claims12
A method of detecting in a sample an antibody specific for a antigenically-active antigen, said method comprising:
- (a) contacting said sample with a protein or peptide comprising an isolated and purified antienically-active Rh antigen protein or peptide, under conditions effective to allow the formation of an immune complex;
- and
- (b) detecting the immune complex so formed.
An immunodetection kit comprising, in suitable container means, a protein or peptide comprising an isolated and purified antigenically-active Rh antigen protein or peptide, and an immunodetection reagent.
Dependencies are inferred from the claim text.