Arrayed primer extension technique for nucleic acid analysis
AssigneeAmersham Pharmacia Biotech AB
InventorPer Johan Ulfendahl
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Abstract
A nucleic acid analysis method is provided which includes the steps of using a primer to amplify the nucleic acid; providing an array of probes, each probe comprising a sequence identical to the primer and an adjacent sequence; applying fragments of the amplifier nucleic acid under hybridisation conditions to the array, effecting enzymatic chain extension of any probe where the adjacent sequence matches that of a hybridised fragment of the amplified nucleic acid; and observing the location of probes of the array while chain extension has taken place.
Claims16
A nucleic acid analysis method which comprises:
- a) using a primer to amplify the nucleic acid,
- b) providing an array of probes in which each probe comprises a primer sequence that is identical to or complementary to the sequence of the primer, and an adjacent sequence which is different in each probe of the array,
- c) applying the amplified nucleic acid from a) under hybridisation conditions to the array of b),
- d) effecting enzymatic chain extension of any probe where the adjacent sequence matches that of the hybridised amplified nucleic acid, and
- e) observing the location of probes of the array where chain extension has taken place in d).
A nucleic acid analysis kit comprising:
- i) a primer for amplifying a nucleic acid, and
- ii) an array of probes in which each probe comprises a primer sequence that is identical to or complementary to the sequence of the primer, and an adjacent sequence which is different in each probe of the array.
Dependencies are inferred from the claim text.