Genetic marker test for lupus
AssigneesCedars-Sinai Medical Center, The Regents of the University of California
InventorsBetty P. Tsao, Rita M. Cantor, Jerome I. Rotter
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Abstract
Disclosed is a genetic testing method for diagnosing systemic lupus erythematosus (SLE) in a human subject. The method is related to amplifying nucleic acids from human tissue samples and analyzing for a variant allele of a gene encoding poly(ADP-ribosyl)transferase expression (PARP), which is diagnostic of SLE or indicates a genetic predisposition for developing SLE. Also disclosed are useful oligonucleotide primers, primer sets and genetic testing kits for detecting a genetic predisposition for developing SLE.
Claims7
A method of detecting a genetic predisposition in a human subject to develop systemic lupus erythematosus (SLE), comprising:
- a) collecting a tissue sample from a human subject;
- b) amplifying nucleic acids from said tissue sample using a forward primer having nucleotide sequence GAT TCC CCA TCT CTC TTT CTT T (SEQ. ID. NO. 1) and a reverse primer having nucleotide sequence AAA TTG TGG TAA TGA CTG CA (SEQ ID. NO. 2) to obtain amplification products, said nucleic acids comprising a genomic sequence of human chromosome lq41-q42 between microsatellite markers D1S2860 and D1S213;
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- c) detecting in the amplification products the presence or absence of a fragment of 85-base pair length, the presence of said fragment of 85-base pair length indicating a genetic predisposition in said subject to develop SLE.
A method of detecting a genetic predisposition in a human subject to develop systemic lupus erythematosus (SLE), comprising the steps of
- a) collecting a tissue sample from a human subject;
- b) amplifying nucleic acids from said tissue sample using a forward primer having a nucleotide sequence GAT TCC CCA TCT CTC TTT CTT T (SEQ. ID. NO. 1) and a reverse primer having a nucleotide sequence AAA TTG TGG TAA TGA CTG CA (SEQ ID. NO. 2) to obtain amplification products, said nucleic acids comprising a genomic sequence of human chromosome lq41-q42 between microsatellite markers D1S2860 and D1S213;
- and
- c) detecting in the amplification products the presence or absence of a fragment of 97-base pair length, the absence of said fragment of 97-base pair length indicating a genetic predisposition in said subject to develop SLE.
An oligonucleotide primer for detecting in a human subject a genetic susceptibility to SLE, said primer comprising the oligonucleotide sequence GAT TCC CCA TCT CTC TTT CTT T (SEQ. ID. NO. 1).
A primer set for detecting in a human subject a genetic susceptibility to SLE, said primer set having a forward primer comprising nucleotide sequence GAT TCC CCA TCT CTC TTT CTT T (SEQ. ID. NO. 1) or a fragment thereof at least 18 nucleotides long, and a reverse primer comprising nucleotide sequence AAA TTO TGG TAA TGA CTG CA (SEQ ID. NO. 2) or a fragment thereof at least 18 nucleotides long.
Dependencies are inferred from the claim text.