Gene expression library produced from DNA from uncultivated microorganisms and methods for making the same
AssigneeDiversa Corporation
InventorJay M. Short
Life of this patent
Estimating the term…
Abstract
Disclosed is a process of screening clones having DNA from an uncultivated microorganism for a specified protein, e.g. enzyme, activity by screening for a specified protein, e.g. enzyme, activity in a library of clones prepared by (i) recovering DNA from a DNA population derived from at least one uncultivated microorganism; and (ii) transforming a host with recovered DNA to produce a library of clones which is screened for the specified protein, e.g. enzyme, activity.
Claims22
A method for identifying an enzymatic activity of interest comprising:
- culturing a gene expression library comprising a pool of expression constructs, each expression construct comprising a vector containing one or more cDNA or genomic DNA fragments, wherein the cDNA or genomic DNA fragments in the pool of expression constructs are derived from a plurality of species of donor eucaryotic organisms, and wherein the cDNA or genomic DNA fragments are each operably-associated with one or more regulatory regions that drives expression of genes encoded by the cDNA or genomic DNA fragments in an appropriate host cell;
- and
- detecting the enzymatic activity encoded by this cDNA or genomic DNA fragments.
A method for identifying an enzymatic activity of interest comprising:
- culturing a gene expression library comprising a pool of expression constucts, each expression construct comprising a vector containing one or more cDNA or gertomic DNA fragments, wherein the cDNA or genomic DNA fragments in the pool of expression constructs are derived from a plurality of species of donor organisms, and wherein the cDNA or genomnic DNA fragments are each operably-associated with one or more regulatory regions that drives expression of genes encoded by the cDNA or genomic DNA fragments in an appropriate host organism, wherein the host cell is a bacterial cell;
- and
- detecting the enzymatic activity encoded by the cDNA or genomic DNA fragments.
A method for identifying an enzymatic activity of interest comprising:
- culturing a gene expression library comprising a pool of expression constructs, each expression construct comprising a vector containing one or more cDNA or genomic DNA fragments, wherein the cDNA or genomic DNA fragments in the pool of expression constructs are derived from a plurality of species of donor organisms, and wherein the cDNA or genomic DNA fragments are each operably-associated with one or more regulatory regions that drives expression of genes encoded by the cDNA or genomic DNA fragments in an appropriate host organism, wherein the host cell is a fungal cell;
- and
- detecting the enzymatic activity encoded by the cDNA or genomic DNA fragments.
A method for identifying an enzymatic activity of interest comprising:
- culturing a gene expression library comprising a pool of expression constructs, each expression construct comprising a vector containing one or more cDNA or genomic DNA fragments, wherein the cDNA or genotnic DNA fragments in the pool of expression constructs are derived from a plurality of species of donor organisms, and wherein the cDNA or genomic DNA fragments are each operably-associated with one or more regulatory regions that drives expression of genes encoded by the cDNA or genomic DNA fragments in an appropriate host organism, wherein the host cell is a plant cell;
- and
- detecting the enzymatic activity encoded by the cDNA or genomic DNA fragments.
A method for identifying an enzymatic activity of interest comprising:
- culturing a gene expression library comprising a pool of expression constructs, each expression construct comprising a vector containing one or more cDNA or genomic DNA fragments, wherein the cDNA or genomic DNA fragments in the pool of expression constructs are derived from a plurality of species of donor organisms, and wherein the cDNA or genomic DNA fragments are each operably-associated with one or more regulatory regions that drives expression of genes encoded by the cDNA or genomic DNA fragments in an appropriate host organism, wherein the host cell is an animal cell;
- and detecting the enzymatic activity encoded by the cDNA or genomic DNA fragments.
Dependencies are inferred from the claim text.