Polyethylene glycol and glutathione composition and method for the treatment of blood vessels prior to cryopreservation
AssigneeOrgan Recovery Systems, Inc.
InventorKelvin G. M. Brockbank
Life of this patent
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Abstract
A composition including polyethylene glycol (PEG) and glutathione (GSH), is used to treat vascular grafts prior to cryopreservation. The PEG and GSH containing composition is use to treat vascular tissue grafts prior to their cryopreservation to ameliorate the onset of intimal hyperplasia. The composition can also be used to treat vascular tissue grafts prior to cryopreservation by incorporation into solutions used for vascular tissue graft transport and/or in other vascular tissue graft processing steps.
Claims30
A method of treating vascular tissue prior to cryopreservation, storage and transplantation, comprising placing the vascular tissue in contact with a first composition containing polyethylene glycol and an antioxidant in amounts effective to inhibit cryopreservation-induced intimal hyperplasia in the vascular tissue upon transplantation, and placing the vascular tissue in contact with a different second composition for cryopreservation.
A method of ameliorating the formation of intimal hyperplasia in vascular grafts, comprising contacting vascular tissue with a first composition comprising polyethylene glycol and glutathione;
- cryopreserving said vascular tissue with a different second composition;
- and transplanting the vascular tissue.
A method, comprising:
- removing vascular tissue from a donor organism;
- contacting for at least about 12 hours the vascular tissue with a first composition comprising between about 5 to about 20% by weight of polyethylene glycol and between about 1 and 100 micromolar amount of glutathione;
- crygopreserving said vascular tissue with a different second composition;
- and
- transplanting the vascular tissue to an organism different from the donor organism.
Dependencies are inferred from the claim text.