Immunomodulating compositions from bile
AssigneeLorus Therapeutics Inc.
InventorRomeo Rang
Life of this patent
Estimating the term…
Abstract
The present invention relates to a composition for use as an immunomodulator comprising small molecular weight components of less than 3000 daltons, and having the following properties: a) is extractable from bile of animals; b) is capable of stimulating monocytes and macrophages in vitro; c) is capable of modulating tumor necrosis factor production; d) contains no measurable IL-1a, IL-1b, TNF, IL-6, IL-8, IL-4, GM-CSF or IFN-gamma; e) has an anti-proliferative effect in a malignant mouse hybridoma cell line; f) shows no cytotoxicity to human peripheral blood mononuclear cells; and g) is not an endotoxin. The invention also relates to a method of preparing the composition and its use an immunomodulator.
Claims7
A process for preparing a composition for use as an immunomodulator comprising small molecular weight components of less than 3000 daltons, and having the following properties:
- a) is extracted from bile of animals;
- b) is capable of stimulating monocytes and macrophages in vitro;
- c) is capable of modulating tumor necrosis factor production;
- d) contains no measurable level of IL-1, IL-1b, TNF, IL-6, IL-8, IL-4, GM-CSF or IFN-gamma;
- e) shows no cytotoxicity to human peripheral blood mononuclear cells;
- and
- f) is not an endotoxin,
- said process comprising (a) mixing bile from an animal with an equal volume of an alcohol to produce a bile/alcohol solution;
- (b) separating out the alcohol soluble fraction and isolating a solution substantially free of alcohol;
- (c) removing bile pigments from the solution to obtain a colorless liquid;
- (d) treating the colorless liquid to substantially remove any residual alcohol;
- (e) extracting the colorless liquid with ether and isolating the aqueous phase;
- and (f) removing residual ether from the aqueous phase.
A process for preparing an immunomodulator composition comprising (a) mixing bile from an animal with a water-soluble solvent to produce a bile/solvent solution;
- (b) isolating an aqueous solution substantially free of solvent from the bile/solvent solution;
- and (c) removing bile pigments from the substantially solvent-free solution to obtain a colorless liquid.
Dependencies are inferred from the claim text.